engleski

Coumarine derivatives as potential acetylcholinesterase inhibitors

Acetylcholinesterase inhibitors have been highly regarded as potential drugs for Alzheimer's disease treatment. Inhibitory properties of AChE inhibitors show great potential as active compounds in plant protection treatments, functioning as pesticides. 38 newly synthesized coumarin derivatives have been tested as potential acetylcholinesterase inhibitors by utilizing the Ellman protocol. The experiment was conducted on a 96-well microplate UV-Vis reader at a wavelength of 412 nm. Ligand-receptor interactions were observed by molecular docking. Inhibition activity results of the 38 compounds were compared to the obtained results of nematicidal activity study against two species of naturally occurring beneficial soil nematodes: Heterorhabditis bacteriophora and Steinernema feltiae. The highest inhibition activity against acetylcholinesterase was obtained by compound 19 (3-cyano-8- hydroxycoumarin) (78.21 % inhibition). The same compound has shown a very small percentage of mortality of nematodes: H. bacteriophora (20.75 %) and S. feltiae (11.25 %). The main interactions with amino acid residues in the binding site of acetylcholinesterase (PDB: 1eve) of compound 19 consists of conventional hydrogen bond (green) ; van der Waals (light green) ; and π–alkyl interactions.

acetylcholinesterase inhibition ; nematodes ; molecular docking

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