engleski

Determination of influential parameters for chitosan adsorption on silica surface

Chitosan, a deacylated derivative of chitin, is a biopolymer composed of two kinds of common saccharides, D-glucosamine and N-acetyl-D- glucosamine. The uniqueness of chitosan lies in its polycationic nature in acidic solutions. Due to its biocompatibility, anti-bacterial/fungal properties, non-toxicity and ease of adaptability to different forms (layers, film, capsules), its main applications are in agriculture, food processing and biotechnology. In this work, we prepared chitosan monolayers on Si-SiO2 substrates using dipping technique starting from chitosan with different molecular weights. Layer properties not only depend on both molecular weight and degree of acetylation of chitosan itself but also on the experimental parameters like solution pH, ionic strength, chitosan concentration and dipping time. Characterization of prepared monolayers was done using ellipsometry and atomic force microscopy (AFM). Ellipsometry was used as a screening method for layer thickness, while AFM was used to image the Si-SiO2 surface, accurately determine layer roughness and thickness and to determine surface coverage with the chitosan layer.

Chitosan ; Polyelectrolyte adsorbtion ; Biocompatibility ; Ellipsometry ; Atomic force microscopy

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