engleski

IN VIVO ANTITUMOR EFFECT OF RESVERATROL NANOCRYSTALS IN MICE AFTER INTRAPERITIONEAL INJECTION

Resveratrol (3, 5, 4′-trihydroxy-stilbene) is a potential antitumor compound because of his antioxidative, anti-inflammatory, anti-angiogenics and anti-proliferation characteristics. Due to poor solubility and bioavailability, his potential therapeutic use is very limited. The aim of this research was to investigate effects of resveratrol nanocrystals on fast-growing and angiogenesis-dependent Ehrlich ascites tumour (EAT) in mice. Furthermore, we investigate the relationship between liver and kidney damage levels through histological analysis of the presence of cells in mitosis, apoptosis and necrosis.The tumour was caused by intraperitoneal (IP) injection of 2.5 x 106 cells into the abdominal cavity of Swiss albino mice. Treatment of animals with EAT tumour in groups was started the next day by IP injection of resveratrol or resveratrol nanocrystals at dose of 25 mg/kg every other day for 14 days. Animals were sacrificed on the 15th day and organs of interest were collected for further analyses.The results indicate that resveratrol nanocrystals have an inhibitory effect on tumour cell growth, but also can induce acute toxicity of the kidney and liver. Microscopic analysis of the number of blood vessels in the peritoneal sheath confirms the great angio-suppressive potential of resveratrol nanocrystals, which in turn leads to inhibition of tumour growth. Resveratrol nanocrystals have shown very promising results as an anti-cancer agent, but the mechanisms of toxicity needs to be further clarified.

resveratrol, nanocrystals, antitumor efficiacy

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