engleski

Micronucleus cytome assay results in obese patients with body mass index (BMI)≥35 on a 500-kcal-3-week diet controlled in hospital

As the country with the highest obesity prevalence in the EU and 5th in the EU according to adult obesity percentage, Croatia requires a systematic and serious approach, especially among the (severely) obese. Previously, and for the first time, our use of a short-term restricted diet in controlled conditions gave good results and kept the interest of the severely obese for life style and dietary habits change with significant improvements in primary DNA damage and oxidative stress measured by the comet assay, and anthropometric and biochemical parameters related to DNA damage and obesity (BMI reduction, 10-35 % excessive weight loss, decrease in TSH, fT3, fT4, basal metabolism rate, waist-hip ratio, visceral fat level, body fat mass, percent body fat, skeletal muscle mass, energy intake, significant decrease in insulin, glucose, homeostatic model assessment for insulin resistance, urea, cholesterol, HDL and LDL levels). We wanted to ascertain whether a similar effect on genomic stability can be seen in permanent DNA damage measured with cytochalasin B-blocked micronucleus cytome assay. Whole blood of 22 volunteers with BMI ≥35 was analysed before and at the end of the 3-week medically-controlled 500 kcal diet (50-60 % complex carbohydrates, 20-25 % protein and 25-30 % fat, carbo-glycaemic index attention). Results demonstrated correlation with oxidatively damaged DNA, significant decrease in micronucleus and nucleoplasmic bridge frequency, nuclear division index and apoptosis. There were correlations with vitamins B12 and D. This approach can lead to significant health improvements and permanent genomic stability in the severely obese.

apoptosis ; calorie restricted diet ; comet assay ; DNA damage ; obesity

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