engleski

Biological activities coumarinyl Schiff bases related to plant protection: Deeper insight into the mechanism of action

Coumarins, secondary plant metabolites[1] and their derivatives demonstrated a wide range of biological activities on different organisms (invertebrate pests, pathogenic fungus and other microorganisms and weeds. The aim was to study the inhibitory effects of coumarinyl schiff bases on the on the four cultures of plant pathogenic fungi (Macrophomina phaseolina, Sclerotinia sclerotiorum, Fusarium oxysporum f. sp. lycopersici, F. culmorum), as well as against two beneficial soil of bacteria (Bacillus mycoides and Bradyrhizobium japonicum). The radial growth of the fungal colonies was measured 48 h after inoculation. Coumarin derivatives were most effective were against M. phaseolina, but were not harmful against beneficial bacteria. The most effective compound against M. phaseolina was 4-bromophenyl-1-p-tolyl-1H-pyrazol derivatives of 2-((4-methyl-2-oxo-2H-chromen-7-yl)oxy)acetohydrazide (4) (71.51 % inhibition). In order to determine the possible mechanism of action of coumarin derivatives against pathogenic fungi, molecular docking study has been performed on three enzymes responsible for the fungal growth: demethylase (pdb ID: 5eah) ; chitinase (pdb ID: 4txe) ; transferase (pdb ID: 2p6g) ; and the three plant cell wall-degrading enzymes: endoglucanase I (pdb ID: 2ovw) ; proteinase K (pdb ID: 2pwb) [53] ; pectinase (pdb ID: 1czf). Analyzed coumarin derivatives are promising candidates for developing plant protection products that could be safe for the environment, human health, and non-target organisms.

coumarins ; Schiff bases ; plant pathogenic fungi ; molecular docking

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