hrvatski

Korelacija kronološke dobi s debljinom celularnog cementa u forenzičnoj stomatologiji

Introduction: Age assessment is an important factor in identifying an individual in the forensic sciences. Previously published studies are scarce, but they have indicated that tooth cement annulations can be used more reliably for assessing dental age than any other morphological or histological feature of an adult skeleton. Aim: The purpose of this study was to examine the correlation between chronological age and cellular cement thickness in male and female subjects. Materials and methods: The study sample consisted of 57 donor teeth of both sexes. The teeth used were extracted because of periodontal disease or for orthodontic or prosthetic reasons. The excluding criterion was the presence of tooth root lesions. Donors’ age ranged from 10 to 69 years at the time of tooth extraction. Teeth with a known chronological age of the donor were divided into three groups: 10 - 19, 30 - 39 and 60 - 69 years, and were classified by sex. Each tooth was embedded in a quick-setting autoacrylate, and the roots were cut with transverse incisions on an ISOMET 1000 cutter in the apical, middle and cervical third of the tooth’s root. The thickness of each incision ranged from 0.3 to 0.5 μm. Cement thickness measurements were made with a light microscope and an Olympus EP50 camera, Version: V3_20190202. The correlation between the achieved estimated dental age, and chronological age was calculated using the Spearman correlation coefficient. Results: A positive correlation was found (r = 0.47, p <0.001) between the estimated and the known chronological age of the donor. The average (median [IQR]) cement thicknesses in the group of 10-19 years was 94.70 [58.17-153.95] µm ; in the group of 30-39 years 231.91 [183.80-332.21] µm ; in the group of 60-69 years 172.29 [135.91- 284.05] µm. Cement thickness decreases from apical to cervical incision (median [IQR] apical 216.72 [128.25-375.00] μm, middle 158.44 [87.66- 284.90] μm ; cervical 96.60 [70 , 05-165.59] µm). It was found that the estimated dental age can be significantly affected by gender (cervical cross section male / female, 174.04 vs. 112.97 µm, t = 2.071, p = 0.043), number of tooth roots (cervical cross section 1 /> 1 root, 105.04 to 167.57 µm, t = 2.166, p = 0.035), and the condition of the tooth crown (cervical section visibly intact / destroyed crown, 123.25 to 218.96, t = 2.116, p = 0.039). The effect of these factors (sex, number of tooth roots, and condition of the tooth crown) on the estimated dental age depends on the place of measurement of the thickness of cellular cementum and is greater on the cervical part. Conclusion: The study shows that the obtained quantitative values of cellular cement thickness can be a reliable tool used in estimating the dental age of humans. As age increases, the thickness of cellular cement increases ; hence the amount of cellular cement can also be used as an indicator of cadaver age in post-mortal forensic analyzes. The thickness of the cellular cementum is greater in men, especially in the cervical third of the tooth root

forensic stomatology ; age assessment ; tooth cement thickness ; transverse incisions

nije evidentirano

engleski

Correlation of chronological age with cellular cement thickness in forensic dentistry

Introduction: Age assessment is an important factor in identifying an individual in the forensic sciences. Previously published studies are scarce, but they have indicated that tooth cement annulations can be used more reliably for assessing dental age than any other morphological or histological feature of an adult skeleton. Aim: The purpose of this study was to examine the correlation between chronological age and cellular cement thickness in male and female subjects. Materials and methods: The study sample consisted of 57 donor teeth of both sexes. The teeth used were extracted because of periodontal disease or for orthodontic or prosthetic reasons. The excluding criterion was the presence of tooth root lesions. Donors’ age ranged from 10 to 69 years at the time of tooth extraction. Teeth with a known chronological age of the donor were divided into three groups: 10 - 19, 30 - 39 and 60 - 69 years, and were classified by sex. Each tooth was embedded in a quick-setting autoacrylate, and the roots were cut with transverse incisions on an ISOMET 1000 cutter in the apical, middle and cervical third of the tooth’s root. The thickness of each incision ranged from 0.3 to 0.5 μm. Cement thickness measurements were made with a light microscope and an Olympus EP50 camera, Version: V3_20190202. The correlation between the achieved estimated dental age, and chronological age was calculated using the Spearman correlation coefficient. Results: A positive correlation was found (r = 0.47, p <0.001) between the estimated and the known chronological age of the donor. The average (median [IQR]) cement thicknesses in the group of 10-19 years was 94.70 [58.17-153.95] µm ; in the group of 30-39 years 231.91 [183.80-332.21] µm ; in the group of 60-69 years 172.29 [135.91- 284.05] µm. Cement thickness decreases from apical to cervical incision (median [IQR] apical 216.72 [128.25-375.00] μm, middle 158.44 [87.66- 284.90] μm ; cervical 96.60 [70 , 05-165.59] µm). It was found that the estimated dental age can be significantly affected by gender (cervical cross section male / female, 174.04 vs. 112.97 µm, t = 2.071, p = 0.043), number of tooth roots (cervical cross section 1 /> 1 root, 105.04 to 167.57 µm, t = 2.166, p = 0.035), and the condition of the tooth crown (cervical section visibly intact / destroyed crown, 123.25 to 218.96, t = 2.116, p = 0.039). The effect of these factors (sex, number of tooth roots, and condition of the tooth crown) on the estimated dental age depends on the place of measurement of the thickness of cellular cementum and is greater on the cervical part. Conclusion: The study shows that the obtained quantitative values of cellular cement thickness can be a reliable tool used in estimating the dental age of humans. As age increases, the thickness of cellular cement increases ; hence the amount of cellular cement can also be used as an indicator of cadaver age in post-mortal forensic analyzes. The thickness of the cellular cementum is greater in men, especially in the cervical third of the tooth root

forensic stomatology ; age assessment ; tooth cement thickness ; transverse incisions

nije evidentirano