hrvatski

Koncentracija fenolnih spojeva i antioksidacijski potencijal klica kupusnjača

Zbog povećane težnje zdravijoj prehrani, interes za svježu, funkcionalnu biljnu hranu raste. Povrće iz porodice kupusnjača (Brassicaceae) bogato je fenolnim spojevima, vitaminima i glukozinolatima, te posjeduje snažan antioksidacijski potencijal što doprinosi pozitivnom djelovanju na ljudski organizam. U posljednje vrijeme sve je učestalija konzumacija klica ove porodice. Klice su mlado tkivo koje je biokemijski vrlo aktivno i u mnogo slučajeva su bogatije bioaktivnim spojevima od zrelih jedinki. Sadržaj tih tvari varira od varijeteta do varijeteta pa su potrebne usporedne analize kako bi se utvrdilo klice kojih varijeteta su bolji izvor određenog bioaktivnog spoja ili biološkog učinka. Cilj ovog rada bio je usporediti koncentracije fenolnih spojeva klica korabice (Brassica oleracea var. acephala gongylodes), kelja (Brassica oleracea sabauda), prokulice (Brassica oleracea gemmifera), cvjetače (Brassica oleracea botrytis), rotkvice (Raphanus sativus) i kreše (Lepidium sativum), te njihov antioksidacijski potencijal kako bi se za svaki od navedenih parametara moglo izdvojiti klice većeg potencijala. Klice su uzgojene u klima komori pri sobnoj temperaturi na vlažnom filter papiru i sakupljene kada su dosegle stadij s dva prava lista. Koncentracija ukupnih fenolnih spojeva nije se značajno razlikovala između analiziranih varijeteta klica. Najbogatije ukupnim flavonoidima bile su klice cvjetače (10, 7 mg ekvivalenata kvercetina/g suhe mase (SM)). Najbogatije ukupnim hidroksicimetnim kiselinama bile su klice kelja (20, 7 mg ekvivalenata cimetne kiseline/g SM) i rotkvice (20, 5 mg ekvivalenata cimetne kiseline/g SM). Najviša koncentracija ukupnih tanina zabilježena je kod klica korabice (4, 0 mg ekvivalenata taninske kiseline/g SM), a najniža kod klica kelja (2, 3 mg ekvivalenata taninske kiseline/g SM). Prema metodi ABTS, sve klice, osim onih rotkvice, imale su veći antioksidacijski potencijal od standardnog antioksidacijskog spoja troloksa koncentracije 7, 5 mg/mL i 50 mg/mL. Prema metodi DPPH, klice kelja, korabice, prokulice i cvjetače imale su antioksidacijski potencijal sličan onome standarda troloksa spomenutih koncentracija. Prema metodi FRAP, sve testirane klice imale su gotovo identičan antioksidacijski potencijal kao standard troloks. S obzirom na rezultate, zaključujemo da klice kupusnjača predstavljaju vrijedan biomaterijal velikog potencijala za ljudsku prehranu i zdravlje, te su daljnje analize njihova biopotencijala itekako potrebne i poželjne. / Due to the increased desire for a healthier diet, interest in fresh, functional plant foods is growing. Vegetables from the cabbage family (Brassicaceae) are rich in phenolic compounds, vitamins and glucosinolates, and have a strong antioxidant potential that contributes to their positive effects on human organism. Lately, the consumption of seedlings of this family has become more frequent. Seedlings are young tissue that is biochemically very active and in many cases are richer in bioactive compounds than mature individuals. The content of these substances varies from variety to variety, so comparative analyzes are needed to determine which seedlings are better source of a particular bioactive compound or biological effect. The aim of this study was to compare the concentrations of phenolic compounds between the seedlings of kohlrabi (Brassica oleracea var. acephala gongylodes), kale (Brassica oleracea sabauda), Brussels sprouts (Brassica oleracea gemmifera), cauliflower (Brassica oleracea botrytis), radish (Raphanus sativus) and cress (Lepidium sativum), and their antioxidant potential so that the seedlings of higher potential could be isolated for each of the analyzed parameter. Seedlings were grown in an air chamber at room temperature on moist filter paper and collected when they reached the two-leaf stage. The concentration of total phenolics did not differ significantly between the analyzed seedling varieties. The highest concentration of total flavonoids had cauliflower seedlings (10, 7 mg quercetin equivalents/g dry weight (DW)). The richest in total hydroxycinnamic acids were seedlings of kale (20, 7 mg of cinnamic acid equivalents/g DW) and radish (20, 5 mg of cinnamic acid equivalents/g DW). The highest concentration of total tannins was recorded in kohlrabi seedlings (4, 0 mg of tannic acid equivalents/g DW), and the lowest in kale seedlings (2, 3 mg of tannin acid equivalents/g DW). According to the ABTS method, all seedlings, except those of radish, had a higher antioxidant potential than the standard antioxidant compound trolox at the concentration of 7, 5 mg/mL and 50 mg/mL. According to the DPPH method, seedlings of cabbage, kohlrabi, Brussels sprouts and cauliflower had an antioxidant potential similar to that of the trolox standard. According to the FRAP method, all tested seedlings had almost identical antioxidant potential as the trolox standard. Given the results, we conclude that Brassicaceae seedlings represent a valuable biomaterial of great potential for human nutrition and health, and further analysis of their biopotential is necessary and desirable.

ABTS, cvjetača, DPPH, flavonoidi, FRAP, hidroksicimetne kiseline, korabica, kelj, kreša, prokulica, rotkvica, tanini

nije evidentirano

engleski

Concentration of phenolic compounds and antioxidant potential of Brassicaceae seedlings

Zbog povećane težnje zdravijoj prehrani, interes za svježu, funkcionalnu biljnu hranu raste. Povrće iz porodice kupusnjača (Brassicaceae) bogato je fenolnim spojevima, vitaminima i glukozinolatima, te posjeduje snažan antioksidacijski potencijal što doprinosi pozitivnom djelovanju na ljudski organizam. U posljednje vrijeme sve je učestalija konzumacija klica ove porodice. Klice su mlado tkivo koje je biokemijski vrlo aktivno i u mnogo slučajeva su bogatije bioaktivnim spojevima od zrelih jedinki. Sadržaj tih tvari varira od varijeteta do varijeteta pa su potrebne usporedne analize kako bi se utvrdilo klice kojih varijeteta su bolji izvor određenog bioaktivnog spoja ili biološkog učinka. Cilj ovog rada bio je usporediti koncentracije fenolnih spojeva klica korabice (Brassica oleracea var. acephala gongylodes), kelja (Brassica oleracea sabauda), prokulice (Brassica oleracea gemmifera), cvjetače (Brassica oleracea botrytis), rotkvice (Raphanus sativus) i kreše (Lepidium sativum), te njihov antioksidacijski potencijal kako bi se za svaki od navedenih parametara moglo izdvojiti klice većeg potencijala. Klice su uzgojene u klima komori pri sobnoj temperaturi na vlažnom filter papiru i sakupljene kada su dosegle stadij s dva prava lista. Koncentracija ukupnih fenolnih spojeva nije se značajno razlikovala između analiziranih varijeteta klica. Najbogatije ukupnim flavonoidima bile su klice cvjetače (10, 7 mg ekvivalenata kvercetina/g suhe mase (SM)). Najbogatije ukupnim hidroksicimetnim kiselinama bile su klice kelja (20, 7 mg ekvivalenata cimetne kiseline/g SM) i rotkvice (20, 5 mg ekvivalenata cimetne kiseline/g SM). Najviša koncentracija ukupnih tanina zabilježena je kod klica korabice (4, 0 mg ekvivalenata taninske kiseline/g SM), a najniža kod klica kelja (2, 3 mg ekvivalenata taninske kiseline/g SM). Prema metodi ABTS, sve klice, osim onih rotkvice, imale su veći antioksidacijski potencijal od standardnog antioksidacijskog spoja troloksa koncentracije 7, 5 mg/mL i 50 mg/mL. Prema metodi DPPH, klice kelja, korabice, prokulice i cvjetače imale su antioksidacijski potencijal sličan onome standarda troloksa spomenutih koncentracija. Prema metodi FRAP, sve testirane klice imale su gotovo identičan antioksidacijski potencijal kao standard troloks. S obzirom na rezultate, zaključujemo da klice kupusnjača predstavljaju vrijedan biomaterijal velikog potencijala za ljudsku prehranu i zdravlje, te su daljnje analize njihova biopotencijala itekako potrebne i poželjne. / Due to the increased desire for a healthier diet, interest in fresh, functional plant foods is growing. Vegetables from the cabbage family (Brassicaceae) are rich in phenolic compounds, vitamins and glucosinolates, and have a strong antioxidant potential that contributes to their positive effects on human organism. Lately, the consumption of seedlings of this family has become more frequent. Seedlings are young tissue that is biochemically very active and in many cases are richer in bioactive compounds than mature individuals. The content of these substances varies from variety to variety, so comparative analyzes are needed to determine which seedlings are better source of a particular bioactive compound or biological effect. The aim of this study was to compare the concentrations of phenolic compounds between the seedlings of kohlrabi (Brassica oleracea var. acephala gongylodes), kale (Brassica oleracea sabauda), Brussels sprouts (Brassica oleracea gemmifera), cauliflower (Brassica oleracea botrytis), radish (Raphanus sativus) and cress (Lepidium sativum), and their antioxidant potential so that the seedlings of higher potential could be isolated for each of the analyzed parameter. Seedlings were grown in an air chamber at room temperature on moist filter paper and collected when they reached the two-leaf stage. The concentration of total phenolics did not differ significantly between the analyzed seedling varieties. The highest concentration of total flavonoids had cauliflower seedlings (10, 7 mg quercetin equivalents/g dry weight (DW)). The richest in total hydroxycinnamic acids were seedlings of kale (20, 7 mg of cinnamic acid equivalents/g DW) and radish (20, 5 mg of cinnamic acid equivalents/g DW). The highest concentration of total tannins was recorded in kohlrabi seedlings (4, 0 mg of tannic acid equivalents/g DW), and the lowest in kale seedlings (2, 3 mg of tannin acid equivalents/g DW). According to the ABTS method, all seedlings, except those of radish, had a higher antioxidant potential than the standard antioxidant compound trolox at the concentration of 7, 5 mg/mL and 50 mg/mL. According to the DPPH method, seedlings of cabbage, kohlrabi, Brussels sprouts and cauliflower had an antioxidant potential similar to that of the trolox standard. According to the FRAP method, all tested seedlings had almost identical antioxidant potential as the trolox standard. Given the results, we conclude that Brassicaceae seedlings represent a valuable biomaterial of great potential for human nutrition and health, and further analysis of their biopotential is necessary and desirable.

ABTS, Brussels sprouts, cauliflower, cress, DPPH, flavonoids, FRAP, hydroxycinnamic acids, kale, kohlrabi, radish, tannins

nije evidentirano