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Pregled bibliografske jedinice broj: 1156489

Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness


Tadijan, Ana; Precazzini, Francesca; Hanžić, Nikolina; Radić, Martina; Gavioli, Nicolò; Vlašić, Ignacija; Ozretić, Petar; Pinto, Lia; Škreblin, Lidija; Barban, Giulia et al.
Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness // Cancers, 13 (2021), 20; 5231, 25 doi:10.3390/cancers13205231 (međunarodna recenzija, članak, znanstveni)


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Naslov
Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness

Autori
Tadijan, Ana ; Precazzini, Francesca ; Hanžić, Nikolina ; Radić, Martina ; Gavioli, Nicolò ; Vlašić, Ignacija ; Ozretić, Petar ; Pinto, Lia ; Škreblin, Lidija ; Barban, Giulia ; Slade, Neda ; Ciribilli, Yari

Izvornik
Cancers (2072-6694) 13 (2021), 20; 5231, 25

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
melanoma ; p53 ; p73 ; Δ160p53 ; isoforms ; targeted therapy ; resistance

Sažetak
Cutaneous melanoma is the most aggressive form of skin cancer. Despite the significant advances in the management of melanoma in recent decades, it still represents a challenge for clinicians. The TP53 gene, the guardian of the genome, which is altered in more than 50% of human cancers, is rarely mutated in melanoma. More recently, researchers started to appreciate the importance of shorter p53 isoforms as potential modifiers of the p53-dependent responses. We analyzed the expression of p53 and p73 isoforms both at the RNA and protein level in a panel of melanoma-derived cell lines with different TP53 and BRAF status, in normal conditions or upon treatment with common anti-cancer DNA damaging agents or targeted therapy. Using lentiviral vectors, we also generated stable clones of H1299 p53 null cells over-expressing the less characterized isoforms Δ160p53α, Δ160p53β, and Δ160p53γ. Further, we obtained two melanoma-derived cell lines resistant to BRAF inhibitor vemurafenib. We observed that melanoma cell lines expressed a wide array of p53 and p73 isoforms, with Δ160p53α as the most variable one. We demonstrated for the first time that Δ160p53α, and to a lesser extent Δ160p53β, can be recruited on chromatin, and that Δ160p53γ can localize in perinuclear foci ; moreover, all Δ160p53 isoforms can stimulate proliferation and in vitro migration. Lastly, vemurafenib-resistant melanoma cells showed an altered expression of p53 and p73 isoforms, namely an increased expression of potentially pro-oncogenic Δ40p53β and a decrease in tumor-suppressive TAp73β. We therefore propose that p53 family isoforms can play a role in melanoma cells’ aggressiveness.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
IP-2013-11-1615 - Otkrivanje novih proteinskih interakcija kao podloga za nove pristupe liječenju melanoma čovjeka (ProNetMel) (Slade, Neda, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Hrvatski zavod za transfuzijsku medicinu

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com fulir.irb.hr

Citiraj ovu publikaciju:

Tadijan, Ana; Precazzini, Francesca; Hanžić, Nikolina; Radić, Martina; Gavioli, Nicolò; Vlašić, Ignacija; Ozretić, Petar; Pinto, Lia; Škreblin, Lidija; Barban, Giulia et al.
Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness // Cancers, 13 (2021), 20; 5231, 25 doi:10.3390/cancers13205231 (međunarodna recenzija, članak, znanstveni)
Tadijan, A., Precazzini, F., Hanžić, N., Radić, M., Gavioli, N., Vlašić, I., Ozretić, P., Pinto, L., Škreblin, L. & Barban, G. (2021) Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness. Cancers, 13 (20), 5231, 25 doi:10.3390/cancers13205231.
@article{article, author = {Tadijan, Ana and Precazzini, Francesca and Han\v{z}i\'{c}, Nikolina and Radi\'{c}, Martina and Gavioli, Nicol\`{o} and Vla\v{s}i\'{c}, Ignacija and Ozreti\'{c}, Petar and Pinto, Lia and \v{S}kreblin, Lidija and Barban, Giulia and Slade, Neda and Ciribilli, Yari}, year = {2021}, pages = {25}, DOI = {10.3390/cancers13205231}, chapter = {5231}, keywords = {melanoma, p53, p73, Δ160p53, isoforms, targeted therapy, resistance}, journal = {Cancers}, doi = {10.3390/cancers13205231}, volume = {13}, number = {20}, issn = {2072-6694}, title = {Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness}, keyword = {melanoma, p53, p73, Δ160p53, isoforms, targeted therapy, resistance}, chapternumber = {5231} }
@article{article, author = {Tadijan, Ana and Precazzini, Francesca and Han\v{z}i\'{c}, Nikolina and Radi\'{c}, Martina and Gavioli, Nicol\`{o} and Vla\v{s}i\'{c}, Ignacija and Ozreti\'{c}, Petar and Pinto, Lia and \v{S}kreblin, Lidija and Barban, Giulia and Slade, Neda and Ciribilli, Yari}, year = {2021}, pages = {25}, DOI = {10.3390/cancers13205231}, chapter = {5231}, keywords = {melanoma, p53, p73, Δ160p53, isoforms, targeted therapy, resistance}, journal = {Cancers}, doi = {10.3390/cancers13205231}, volume = {13}, number = {20}, issn = {2072-6694}, title = {Altered Expression of Shorter p53 Family Isoforms Can Impact Melanoma Aggressiveness}, keyword = {melanoma, p53, p73, Δ160p53, isoforms, targeted therapy, resistance}, chapternumber = {5231} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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