Application of MALDI-TOF-MSI method on 3D cell models (CROSBI ID 710075)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Filipović, Nikolina ; Marković, Berislav ; Debeljak Željko ; Mišković Špoljarić, Katarina
engleski
Application of MALDI-TOF-MSI method on 3D cell models
Mass spectrometry with imaging (MSI) is an extremely powerful method that, in addition to being a research analytical technique, can also be used for targeted detection of significant molecular species. Unlike classical visualization methods, by using MSI technique, it is possible to identify hundreds of analytes simultaneously. The addition of TOF (Time of Flight) allows ion flight time to differentiate according to the value of ion mass/charge. Consequently - ions with lower m/z values travel faster. Characteristics such as the ability to scan a sample up to 20 times make this technique excellent for monitoring and reviewing the spatial distribution of different molecular species in many cell and tissue structures. Cells grown as 3D cultures are very attractive model for study in biological researches. The reason for this is the greater compatibility with in vivo conditions, since cells are cultured in the form of the spheroids whose microenvironment is similar to that in living tissues.1-2 The above method with all its advantages will be used to detect the effect of organometallic compounds on 3D models of ¬¬¬Caco- 2 and Hep-G2 cell lines.
spectroscopy, 3D cell model, MALDI, TOF-MSI, organometallic compounds
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Podaci o prilogu
188-188.
2021.
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objavljeno
Podaci o matičnoj publikaciji
Book of abstracts 27th Croatian Meeting of Chemists and Chemical Engineers
Podaci o skupu
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)
poster
05.10.2021-08.10.2021
Veli Lošinj, Hrvatska