engleski

REGULATORY T-LYMPHOCYTES IN LYMPHOMAGENESIS

B-cell lymphomas are a heterogenous group of tumours that account for 85% of all non-Hodgkin lymphomas (NHL), tumours derived from cells of lymphoid lineage. B-lymphomas originate from various developmental stages of B lymphocytes. Classical Hodgkin’s lymphoma (cHL) also originates from B lymphocytes. Helper T lymphocytes expressing the transcription factor FOXP3 (CD4+ FOXP3+), also known as regulatory T lymphocytes (Tregs), are a component of tumour microenvironment (TME). FOXP3 regulates expression of various genes in Tregs responsible for the maintaining of immune tolerance and homeostasis of the immune system. It has been shown that Tregs influence the behaviour of tumour cells. The aim of this study was to evaluate the importance of Tregs in the most common groups of Blymphomas (follicular lymphoma (FL), mantle cell lymphoma (MCL), diffuse large B cell lymphoma (DLBCL)) and in classical Hodgkin’s lymphoma. Origin of the tumour cells was determined by the detection of markers like CD20 by immunohistochemical staining in formalin- fixed, paraffinembedded tissue samples. The same method was applied to evaluate the contents of TME based on markers CD68, CD3, CD4, CD8, PD-1, FOXP3, CD14, CD21. Statistically significant correlation between the numbers of Tregs and macrophages in TME was observed in MCL. Inverse correlation between the numbers of Tregs and cells expressing PD-1 was observed in cHL. No statistically significant correlation between the number of Tregs and the survival of the patients was observed in studied types of B-cell originated lymphoma. These results suggest that Tregs affect the contents of TME in Bcell originated lymphoma.

B lymphoma, transcriptional factor FOXP3, tumour microenvironment

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