engleski

Does storage of blood in EDTA tube affect hematologic parameters or not?

The complete blood count (CBC) provides crucial data for clinicians. The storage conditions after blood collection are an important step in the preanalytical phase and errors can lead to false results. The purpose of this study was to document which parameters of CBC are most affected by storage. Thirteen dogs in random order were included in the study. Blood samples were collected from the cephalic vein into EDTA tubes. Hematological analysis was performed using the Horiba Scil Vet ABC Plus analyzer (Scil, Germany) within one hour of collection. After the initial analysis, 1 mL of the blood was aliquoted and stored at 4 °C, while the remaining blood was stored at room temperature (RT). The CBC analysis was repeated after 24, 48, 72 and 96 hours. Blood stored at 4 °C was mixed for 20 minutes on room temperature before analysis. Leukocytes, hemoglobin (HGB), erythrocytes and hematocrit (HCT) did not change (p> 0.05) from the initial analysis and differed less than 5% during the studied period at both temperatures. MCV was higher at 96 hours post blood collection in samples stored at 4 °C and at 72 and 96 hours stored at RT compared to the initial values (p< 0.05). Red blood cell distribution width was increased at 48, 72 and 96 hours in samples stored at 4 °C and at 72 and 96 hours in samples stored at RT (p< 0.05). MCHC was decreased at 96 hours post-extraction in samples stored at RT (p< 0.05), but remained stable in samples stored at 4 °C during the studied period. Platelet counts decreased during the study from 5% to 45% compared to the initial measurement (p>0.5) with more pronounced decrease in refrigerated samples. Based on our results, leukocytes, erythrocytes, HGB, and HCT could be determined in whole blood stored up to 4 days after collection. When stored in EDTA, erythrocytes swell, which may result in a falsely high MCV and consequently low MCHC. A decreased platelet count during storage could be explained by platelet aggregation at low temperatures.

canine blood ; blood storage ; hematological parameters

nije evidentirano