engleski

Is it possible to use EDTA plasma as a sample for biochemical analyses in veterinary laboratory diagnostics?

The choice of anticoagulant is an important preanalytical factor in laboratory diagnosis. Errors in anticoagulant selection can be avoided or reduced if it is found to affect certain biochemical analytes. The aim of this study was to compare values of some biochemical parameters in dog EDTA plasma and serum samples. Paired plasma (EDTA anticoagulant) and serum samples were collected from cephalic vein of 15 dogs. Serum and EDTA plasma samples were obtained after centrifugation at 1600 g for 10 min. Concentrations of urea, creatinine, total proteins, albumin, glucose, bilirubin, and C-reactive protein (CRP), and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), and creatine kinase (CK) were measured in the paired plasma and serum samples with Abbott reagents on the Abbott Architect Plus c4000 biochemical analyser. The differences in concentrations of urea, creatinine, total proteins and albumin, and in activities of AST, ALT and GGT between serum and EDTA plasma were less than 5%. Glucose concentrations were up to 40% lower in EDTA plasma than in serum. Bilirubin concentrations were lower in EDTA plasma than in serum (16-30%). Alkaline phosphatase activity was significantly lower in EDTA plasma (78-95 %) than in serum. Activity of CK ranged from -18% to +68% in EDTA plasma compared with serum. Concentrations of CRP in EDTA plasma samples were higher than in serum (38-80%) in samples where the CRP was less than 10 mg/L. When the CRP concentration was above 23 mg/L, the CRP differences in EDTA plasma vs. serum were up to 11%. In conclusion, urea, creatinine, total proteins and albumin, and in AST, ALT and GGT can be reliably assayed from either serum or plasma samples in dog samples. Glucose, bilirubin AP, CK should always be assayed in serum. The determination of CRP in EDTA plasma can provide an approximate result, but the control samples must be repeated in EDTA plasma.

EDTA plasma ; biochemical analysis ; veterinary laboratory diagnostics

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