DNA isolation of Chara fragilis Desv. (CROSBI ID 490076)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Liber, Zlatko ; Plenković-Moraj, Anđelka ; Gligora, Marija ; Katić, Iva
engleski
DNA isolation of Chara fragilis Desv.
The molecular-biological research has been successfully applied to systematic and phylogeny of plants. Unfortunately, algae have been researched extremely rare in this way. In this research we tried to find out the relevant method for DNA isolation of Chara fragilis Desv. For this purpose modification of Random Amplified Polymorphic DNA (RAPD) technique was used. The isolation of DNA was conducted on the fresh, frozen (-20 oC) and material stored in 95 % ethanol. Isolation from the frozen material and the material that was stored in 95% ethanol did not give satisfactory results. The DNA was successfully isolated, and RAPD technique was efficacy applied on the fresh material. Three of nine tested primers (OPB-11, OPB-12, OPB-18) could be used in prospective systematic and ecological research among the species of Chara genus.
Chara fragilis Desv.; RAPD method
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
119-x.
2002.
objavljeno
Podaci o matičnoj publikaciji
1st Croatian Congress on Molecular Life Sciences
Dumić, Jerka i dr.
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu
Podaci o skupu
1st Croatian Congress on Molecular Life Sciences
poster
09.06.2002-14.06.2002
Opatija, Hrvatska