engleski

The impact of breastfeeding on the composition of the infant faecal microbiome

Breast milk not only provides a perfect balance of nutrients to meet all the needs of the infant in the first months of life, but also contains a variety of bacteria that play a key role in tailoring the neonatal gut microbiome. To investigate the impact of breast milk on the composition of the infant faecal microbiome, DNA was extracted from samples of breast milk (n=15) and infant faeces (n=15) collected from 5 mother-infant pairs at 3 different time points. The V1-V3 region of the 16S rRNA gene was amplified, and amplicon sequencing was performed using the Illumina MiSeq platform. Analysis of the raw sequencing data using the QIIME 2 platform revealed the unique composition of each breast milk and infant feaces microbiome and confirmed the correlation of their composition in each mother-infant pair. Firmicutes (64.28%) and Proteobacteria were the most predominant phyla in breast milk microbiome, whereas Firmicutes (32.92%) and Actinobacteria (31.86%) dominated the faecal microbiome. The analysis confirmed that the composition of the breast milk microbiome changes during lactation, as does the faecal microbiome during infant growth and development. α-diversity analysis revealed that the breast milk microbiota is more diverse, i.e. species-rich, than the infant faecal microbiota.

breast milk ; microbiome sequencing ; α-diversity ; β-diversity

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