engleski

DEVELOPMENT OF ELECTROCHEMICAL SENSORS FOR DETERMINATION OF VITAMIN C AND EVALUATION OF ANTIOXIDANT ACTIVITY

It is known that carbon materials due to low ohmic resistance can significantly improve the electroanalytical properties of the sensing layers. Carbon materials also provide a variety of modification options that often have been used to develop highly sensitive sensors to investigate electrochemical properties and detect organic and inorganic compounds. The first goal of this work was to develop a simple electrochemical method for the determination of Vitamin C (VitC). The cyclic voltammetry was used to characterize microelectrodes and square wave voltammetry to quantify VitC. A procedure for quantifying VitC in the real sample is established. The second goal was to determine the effect of the addition of a different type of “green” biowaste on plant growth, VitC content, and antioxidant activity in arugula (Eruca sativa L) by using carbon microelectrode. The obtained results were compared with the standardized methods. After three weeks of cultivation, small differences in growth and large differences in certain nutritional characteristics were observed. The addition of peanut shell contributes to soil aeration and the fastest development of healthy and green Eruca sativa has been observed. The addition of black coffee makes the soil slightly alkaline and results in a significant increase in the VitC content and antioxidant activity.

carbon microelectrodes, “green” biowaste, vitamin C, antioxidant activity

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