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Pregled bibliografske jedinice broj: 1154819

Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients


Šeba, Tino; Friganović, Tomislav; Weitner, Tin
Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients, 2020. (ostalo).


CROSBI ID: 1154819 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients

Autori
Šeba, Tino ; Friganović, Tomislav ; Weitner, Tin

Vrsta, podvrsta
Ostale vrste radova, ostalo

Godina
2020

Ključne riječi
transferrin, absorbance, molar absorption coefficient, guanidine

Sažetak
Absorbance of a protein around 280 nm depends on its tryptophan (Trp), tyrosine (Tyr) and cystine (i.e. the disulfide bond) content. Molar absorption coefficient of a folded (native) protein can be determined by measuring absorbance of two protein solutions with identical concentrations, one containing only buffer and the other containing the same buffer and 6.0 M guanidine∙HCl. Since molar absorption coefficients of Trp, Tyr and cystine in 6.0 M guanidine∙HCl are known, molar absorption coefficient of an unfolded (denatured) protein at given wavelength can be calculated if the protein amino acid sequence and structure (i.e. the protein Trp, Tyr and cystine content) is known and the absorbances of the folded and unfolded protein are measured. The molar absorption coefficient of a folded protein at given wavelength is then equal to the product of a molar absorption coefficient of an unfolded protein and absorbance ratio of folded and unfolded protein at given wavelength. The required amount of the protein is 1 mg but approximately 60 % of folded protein can be recovered and used for further studies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( POIROT)

Profili:

Avatar Url Tin Weitner (autor)

Avatar Url Tomislav Friganović (autor)

Avatar Url Tino Šeba (autor)

Poveznice na cjeloviti tekst rada:

doi.org

Citiraj ovu publikaciju:

Šeba, Tino; Friganović, Tomislav; Weitner, Tin
Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients, 2020. (ostalo).
Šeba, T., Friganović, T. & Weitner, T. (2020) Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients.. Ostalo.
@unknown{unknown, author = {\v{S}eba, Tino and Friganovi\'{c}, Tomislav and Weitner, Tin}, year = {2020}, keywords = {transferrin, absorbance, molar absorption coefficient, guanidine}, title = {Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients}, keyword = {transferrin, absorbance, molar absorption coefficient, guanidine} }
@unknown{unknown, author = {\v{S}eba, Tino and Friganovi\'{c}, Tomislav and Weitner, Tin}, year = {2020}, keywords = {transferrin, absorbance, molar absorption coefficient, guanidine}, title = {Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients}, keyword = {transferrin, absorbance, molar absorption coefficient, guanidine} }




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