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Pregled bibliografske jedinice broj: 1154815

Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin


Štambuk, Tamara; Cvetko, Ana; Weitner, Tin
Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin, 2020. (ostalo).


CROSBI ID: 1154815 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin

Autori
Štambuk, Tamara ; Cvetko, Ana ; Weitner, Tin

Vrsta, podvrsta
Ostale vrste radova, ostalo

Godina
2020

Ključne riječi
transferrin ; N-glycan analysis ; UPLC ; mass spectrometry

Sažetak
This protocol describes the method used for N- glycan analysis of the native and desialylated human apo-transferrin. The protein N-glycans were released with the addition of 1.2 U of PNGase F and overnight incubation at 37°C. The released N-glycans were labeled with 2-AB and purified using hydrophilic interaction liquid chromatography solid-phase extraction (HILIC-SPE). Fluorescently labeled N-glycans were separated by UPLC using Waters BEH Glycan chromatography column. All glycan structures were annotated with MS/MS analysis using an ESI-QTOF-MS system

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( POIROT)

Profili:

Avatar Url Ana Cvetko (autor)

Avatar Url Tamara Štambuk (autor)

Avatar Url Tin Weitner (autor)

Poveznice na cjeloviti tekst rada:

doi.org

Citiraj ovu publikaciju:

Štambuk, Tamara; Cvetko, Ana; Weitner, Tin
Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin, 2020. (ostalo).
Štambuk, T., Cvetko, A. & Weitner, T. (2020) Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin.. Ostalo.
@unknown{unknown, author = {\v{S}tambuk, Tamara and Cvetko, Ana and Weitner, Tin}, year = {2020}, keywords = {transferrin, N-glycan analysis, UPLC, mass spectrometry}, title = {Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin}, keyword = {transferrin, N-glycan analysis, UPLC, mass spectrometry} }
@unknown{unknown, author = {\v{S}tambuk, Tamara and Cvetko, Ana and Weitner, Tin}, year = {2020}, keywords = {transferrin, N-glycan analysis, UPLC, mass spectrometry}, title = {Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin}, keyword = {transferrin, N-glycan analysis, UPLC, mass spectrometry} }




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