engleski

Effects of subchronic exposure of rat to individual and combined ochratoxin A and citrinin on the expression of renal water channels and sodium-glucose cotransporters

Ochratoxin A (OTA) and citrinin (CIT) are nephrotoxic mycotoxins that frequently co-contaminate food and feed, thus representing a serious health threat to humans and animals. Studies have shown that these toxins deregulate an array of genes/proteins leading to their expressional changes. However, individual and combined effect of OTA and CIT on the expression of renal proteins facilitating the transport of biologically important molecules such as water and glucose, are poorly studied. In this study, we used immunochemical methods (immunofluorescence microscopy and Western blotting) to reveal the individual and combined effects of OTA (0.125 and 0.250 mg/kg b.w.) and CIT (20 mg/kg b.w.) on the localization/expression of renal water channels (rAqp1 & rAqp2) and sodium-glucose cotransporters (rSglt1 & rSglt2) after a 21-day subchronic oral exposure of Wistar male rats. Additionally, we investigated if antioxidant resveratrol (RSV ; 20 mg/kg b.w.) can counteract the OTA- and CIT-related nephrotoxic effects on the localization/expression of studied proteins. In the rat kidney, the higher individual doses of OTA significantly downregulated protein expression of rAqp1 glycosylated isoforms and upregulated protein expression of rSglt1, whereas combination of higher OTA dose and CIT downregulated expression of rAqp1 non-glycosylated isoforms. Interestingly, the protein expression of rAqp1 glycosylated isoforms and rSglt1 was significantly downregulated in the kidneys of rats exposed to the combination of both mycotoxins (OTA+CIT) and RSV. Thus, OTA and CIT seem to have a selective effect on protein expression of studied transporters in rat kidneys, whereas RSV does not counteract their nephrotoxic effects. (Croatian Science Foundation project IP-09-2014-5982).

kidney ; mycotoxins ; oral exposure ; proximal tubules ; resveratrol ; Wistar rat

nije evidentirano