engleski

Serum proteome profiling in canine babesiosis using a label-based quantitative proteomics approach

Introduction: Babesiosis is a disease of significant veterinary importance and emerging vector‐ borne zoonosis with worldwide distribution. It is caused by the intra-erythrocyte protozoal parasites, with Babesia rossi being the most severe of all the large Babesia parasites infecting dogs. The disease can be clinically classified into uncomplicated and complicated forms with a wide range of clinical presentations from a mild, subclinical illness to complicated forms and death. The aim of this study was to assess serum proteomic profile from dogs with babesiosis and healthy dogs using label based proteomics approach. Materials and methods: In this study 32 dogs naturally infected with B. rossi and 20 healthy dogs were included. Diseased dogs were subdivided into 18 uncomplicated cases and 14 complicated cases of babesiosis. Serum samples were processed using Tandem Mass Tag (TMT) label-based quantitative approach. High resolution LC-MS/MS analysis was carried out using nanoLC system coupled to Q Exactive Plus mass spectrometer. Protein identification and quantification were performed using Proteome Discoverer. Statistical and bioinformatic analysis were done using R and Cytoscape. Results: Altogether, 311 quantifiable proteins were identified (2 unique peptides, 5% FDR). There were 75 master serum proteins with statistically different abundance between the dogs with babesiosis and healthy dogs, including retinol binding protein 4, alpha-1-acid glycoprotein 1- like, vitamin D binding protein, apolipoprotein E, transferrin receptor, paraoxonase and others.Conclusion: Shotgun TMT-based high- resolution proteomic profiling allowed identification of potential serum biomarkers for differentiation of disease severity in canine babesiosis caused by B. rossi. These findings may be applicable to the understanding and study of haemoprotozoal diseases

babesiosis ; quantitative proteomics ; serum

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