engleski

Influence of different rodent models of nonalcoholic fatty liver disease on metabolism of important polyunsaturated fatty acids and inflammation

Metabolic disease, such as nonalcoholic fatty liver disease (NAFLD), is characterized by simple to severe steatosis, and consequent hepatitis, fibrosis, and cirrhosis. Furthermore, it is related to an increase of the inflammatory markers, as well as changes in the content of tissue fatty acids, and their corresponding ratios. High-fructose diet and cafeteria diet are widely used dietary rodent models of NAFLD which involve obesity, glucose intolerance, and inflammation. The aim of our investigation was to determine differences between these two models in the metabolism of important fatty acids and inflammatory markers in the liver and white adipose tissue (WAT). Thirty Wistar rats (15 male and 15 female) were divided into the control group (CON), the high fructose group (HF, 15% of fructose in drinking water) and the cafeteria dietary group (CAF, 50% basal diet and 50% cafeteria diet). The rats were sacrificed after 20 weeks of dietary treatment, and hepatic and WAT samples were obtained. The expressions of inflammation gene markers was quantified by RT-qPCR. The analysis of hepatic and WAT fatty acid composition was performed by gas chromatography. For statistical data analysis, GraphPad 8 was used. The hepatic and WAT fatty acid profile showed significant variations depending on the diet and sex. Generally, in the treated rats significant increase in monounsaturated fatty acids and decrease in n3 and n6 polyunsaturated fatty acids (PUFAs) was observed. The CAF diet induced more pronounced decrease in both n3 and n6 PUFAs. In both diets, the concentration of n6 docosapentaenoic (n6 DPA) acid was increased. The expression of the inflammation gene markers (TGFβ, TNFα and IL6) was significantly increased in both diets and sexes. Two dietary models of NAFLD led to characteristic changes in the hepatic and white adipose tissue fatty acid profiles, in comparison to the control group and to each other. These findings could play an important role in the interpretation of the experimental results.

NAFLD, WAT, liver, inflammation

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