engleski

The Effect of Bioactive Coatings on Enamel Microhardness

Objectives: The aim of this study was to determine the effect of three different varnish materials, containing casein phosphopeptide-amorphous calcium phosphate (CPP- ACP), nano-hydroxyapatite and fluoride respectively, on enamel microhardness. Methods: A total of 30 extracted human third molars were obtained, in order to prepare enamel specimens. They were immersed into autoploymerising acrylic resin and polished, exposing an enamel surface. The specimens were demineralized by applying 37% phosphoric acid for three minutes. Three experimental groups (n=10) were treated with 3M™ Clinpro™ White Varnish, MI Varnish® and Megasonex® toothpaste respectively at every 24 hours for 14 days, using micro brush. Between the treatments the samples were kept in saline. The microhardness of the specimen surface was determined by the Vickers method in triplicate (HV0, 1), in three stages ; baseline value, after demineralization and after the period of remineralization. Data were subjected to Scheffe posthoc test. Results: The mean microhardness values before demineralization, after demineralization, and after remineralization for enamel were 366, 0±18, 93, 190, 30 ± 27, 10, 236, 57 ± 19, 42 for M™ Clinpro™ White Varnish ; 343, 52 ± 26, 66, 192, 73 ± 16, 37, 286, 65 ± 34, 71 for MI Varnish® and 393, 05 ± 16, 14, 201, 90 ± 15, 30, 237, 97 ± 32, 52 for Megasonex®. Microhardness values obtained for samples treated with MI Varnish® were higher compared to the other two (p = 0.001 for both comparisons), while the first and third group did not differ significantly from each other (p = 0.97). Conclusions: The usage of MI Varnish® leads to higher microhardness values of the enamel after demineralization has occurred, compared to 3M™ Clinpro™ White Varnish and Megasonex® toothpaste.

bioactivity ; fluorides ; CPP-ACP ; nano-hydroxyapatite

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