engleski

Planning of gluten free diet using nutritional systems biology approach

Celiac disease is an autoimmune disease that occurs in people with genetic predisposition, where gluten ingestion causes damage to the small intestines.When people with celiac disease eat gluten (a protein found in wheat, ryeand barley), their body has an immune response that attacks the small intestine resulting in damage to the villi in small intestine. When the villi gets damaged, nutrients cannot be properly absorbed into the body. So it is very important to know what happens in the cells after introducing gluten into organism. Lately, it has been recognised that systems biology tools have potential to increase understanding of how nutrition influences metabolic pathways and homeostasis. In this work the effect of the diet of paediatric patients on the celiac disease immune response was analysed using nutritional system biology approach. Celiac disease immune response mathematical model was constructed and analysed using CellDesigner 4.0 (Systems Biology Institute (SBI), Tokyo, Japan). Analysed mathematical model in the form of ordinary differential equations describes processes that take place in two intestinal compartments (lumen and lamina propria) and incorporates 16 variables and 34 processes, which correspond to 34 reaction rates. The effect of the different concentrations of gluten daily intake between paediatric patients on the antibodies level changes was analysed. Western diet consists of 10–20 grams of gluten per day and as ‘safe’ is considered anything under 10 mg per day what is an equivalent to 1/350 of a piece of bread. Atypicalgluten-free dietwillconsistanywherebetween 6 milligramsand 10 mg ofglutenperday but ‘gluten-free’ diet is rarely 100% without gluten i.e. proteins of plant origin from oats, rye, barley and wheat. Mathematical simulations of the celiac disease immune response showed the differences in antibodies levels changes depending on the amount of gluten consumed by paediatric patients. The profile of the antibodies levels decrease after of changing diet form gluten containing to gluten free was also presented. Application of the nutritional systems biology approach in diet planning ensures detail insight in metabolic process and simple control of the metabolic reaction influenced by nutrient intake.

celiac disease ; cell designer ; mathematical model

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