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Pregled bibliografske jedinice broj: 1150504

The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin


Borko, Valentina; Friganović, Tomislav; Weitner, Tin
The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin // 27HSKIKI Abstracts
Veli Lošinj, Hrvatska, 2021. str. 269-269 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 1150504 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin

Autori
Borko, Valentina ; Friganović, Tomislav ; Weitner, Tin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27HSKIKI Abstracts / - , 2021, 269-269

Skup
27th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Veli Lošinj, Hrvatska, 5-8.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
human serum transferrin ; synergistic anion ; thermodynamic parameters ; binding constants ; binding enthalpy

Sažetak
Human transferrin is a serum glycoprotein that has ability to bind, transport and release ferric ions with two binding sites for metal ions, the C-site and N-site. Each bound metal ion is hexa- coordinated: four ligands to the metal are provided by the transferrin amino acid residues, while the remaining two are provided by an external anion, termed the synergistic anion. This synergistic anion is carbonate in vivo, however, other anions (such as oxalate, pyruvate, glycine, etc.) with similar structure can substitute for carbonate in vitro. In this study we determined the thermodynamic parameters for the binding of ferric ion to human serum transferrin in the presence of different synergistic anions (carbonate and oxalate) using isothermal titration calorimetry (ITC). Resulting ITC curves displayed two inflection points typical for a model of two binding sites. Data analysis of the curves was based on the model of two sets of independent sites and best-fit parameters were calculated using Marquardt algorithm. Different slope in the two binding regions on the titration curve can be attributed to different binding affinities of ferric ion to human serum transferrin in the presence of different synergistic anions, suggesting lower affinities in the presence of oxalate. Also, the observed differences in the heat released in the first part of titration suggest different binding enthalpy for the C-site of transferrin. Similar effect on the two binding sites having different metal binding properties in the presence of different synergistic anions is known.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Tin Weitner (autor)

Avatar Url Tomislav Friganović (autor)

Avatar Url Valentina Borko (autor)


Citiraj ovu publikaciju:

Borko, Valentina; Friganović, Tomislav; Weitner, Tin
The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin // 27HSKIKI Abstracts
Veli Lošinj, Hrvatska, 2021. str. 269-269 (poster, domaća recenzija, sažetak, znanstveni)
Borko, V., Friganović, T. & Weitner, T. (2021) The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin. U: 27HSKIKI Abstracts.
@article{article, author = {Borko, Valentina and Friganovi\'{c}, Tomislav and Weitner, Tin}, year = {2021}, pages = {269-269}, keywords = {human serum transferrin, synergistic anion, thermodynamic parameters, binding constants, binding enthalpy}, title = {The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin}, keyword = {human serum transferrin, synergistic anion, thermodynamic parameters, binding constants, binding enthalpy}, publisherplace = {Veli Lo\v{s}inj, Hrvatska} }
@article{article, author = {Borko, Valentina and Friganovi\'{c}, Tomislav and Weitner, Tin}, year = {2021}, pages = {269-269}, keywords = {human serum transferrin, synergistic anion, thermodynamic parameters, binding constants, binding enthalpy}, title = {The effect of oxalate on the thermodynamic parameters for the binding of iron to human serum transferrin}, keyword = {human serum transferrin, synergistic anion, thermodynamic parameters, binding constants, binding enthalpy}, publisherplace = {Veli Lo\v{s}inj, Hrvatska} }




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