Insights on toxicity and detoxication of organophosphorus pesticides in vitro (CROSBI ID 708489)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Čadež, Tena ; Zandona, Antonio ; Katalinić, Maja ; Kovarik, Zrinka
engleski
Insights on toxicity and detoxication of organophosphorus pesticides in vitro
Toxic organophosphorus (OP) pesticides are still widely used in agriculture and are the cause of great risk of exposure for people. Such exposure can lead to distinct neurotoxic effects depending on dose and frequency. The main mechanism of action of OP pesticides is inhibition of cholinergic enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) causing accumulation of neurotransmitter acetylcholine in the synaptic cleft inducing cholinergic crisis. This progressive inhibition occurs by phosphylation of their active centre leading to an inactive enzyme species. After exposure, to prevent cholinergic crisis quick dephosphylation of AChE’s active centar needs to be done by a strong nucleophile with an oxime group to avoid severe health effects and illnesses, ranging from respiratory problems, muscle twitches, convulsions etc. We evaluated the in vitro inhibition rate of AChE by methamidophos and the potency of three pyridinium oximes, 2-PAM analogous, to reactivate OP-inhibited AChE and BChE. The overall inhibition rate of AChE for methamidophos was 2.760 ± 119 min-1 L/mol, which was almost 10-fold higher than for BChE (386 ± 16 min-1 L/mol). The experiments showed that the selected oximes possessed higher efficiency than the standard oxime 2-PAM. Furthermore, an absence of cytotoxicity on human astrocytoma (1321N1) and human liver (HepG2) cells makes these oximes good candidates for future research as reactivators of OP-pesticide inhibited cholinesterases.
Methamidophos, fenamiphos, cholinergic crisis, acetylcholinesterase, butyrylcholinesterase, 2-PAM
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Podaci o prilogu
46-46.
2021.
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objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju
0004-1254
1848-6312
Podaci o skupu
6th Croatian congress of toxicology with international participation (CROTOX 2021)
poster
03.06.2021-06.06.2021
Rabac, Hrvatska
Povezanost rada
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Kemija