engleski

Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review

Matrix-assisted laser desorption/ionization time- of-flight mass spectrometry(MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environ- mental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDITOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have onMALDITOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.

Bacteria ; Culture conditions ; Diagnostics ; MALDI-TOF MS ; Sample preparation

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