engleski

Production of hydrolytic and lignolytic enzymes during cultivation of Trametes versicolor on brewer's spent grain

Brewer’s spent grain (BSG) is a by-product of the brewing industry produced in large quantities worldwide. It plays an important role in the circular bioeconomy based on innovative solutions for waste utilization in the new production cycles. In this work, BSG was used as a substrate for the production of hydrolytic enzymes (xylanase, invertase, β-glucosidase, cellulase) and lignolytic enzymes (laccase, manganese peroxidase, lignin peroxidase) by fungal solid- state fermentation by Trametes versicolor. Fermentation was carried out for 15 days and enzyme activities were measured daily. T. versicolor proved to be a good producer of xylanase (V.A. = 514 U/mL, t = 15 d), invertase (V.A. = 225 U/mL, t = 10 d) and β-glucosidase (V.A. = 4.4 U/mL, t = 12 d), while low activities of cellulase were detected (V.A. = 0.079 FPU/mL, t = 10 d). The highest activities of laccase (V.A. = 174 U/L) and manganese peroxidase (V.A. = 140 U/L) were detected after the 10th day of fermentation, while lignin peroxidase activity was below the measurability limit. Chemical analysis of the BSG after treatment shows its high potential for application in food and feed industry, biogas production or production of value-added products (lactic acid, xylitol, ferulic acid, p-coumaric acid, poly-3-hidroxybutyrate, natural red pigment, ascorbic acid, citric acid, volatile fatty acid, cordycepin).

brewer's spent grain ; hydrolytic enzymes ; lignolytic enzymes ; trametes versicolor

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