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Pregled bibliografske jedinice broj: 1150019

Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles


Ivec, Arian; Trupinić, Monika; Tolić, Iva M.; Pavin, Nenad
Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles // Biophysical journal, 120 (2021), 17; 3641-3648 doi:10.1016/j.bpj.2021.07.024 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1150019 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles

Autori
Ivec, Arian ; Trupinić, Monika ; Tolić, Iva M. ; Pavin, Nenad

Izvornik
Biophysical journal (0006-3495) 120 (2021), 17; 3641-3648

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mitotic spindle ; chirality ; twist ; helicity ; methods

Sažetak
The highly ordered spatial organization of microtubule bundles in the mitotic spindle is crucial for its proper functioning. The recent discovery of twisted shapes of microtubule bundles and spindle chirality suggests that the bundles extend along curved paths in three dimensions, rather than being confined to a plane. This, in turn, implies that rotational forces, i.e., torques, exist in the spindle in addition to the widely studied linear forces. However, studies of spindle architecture and forces are impeded by a lack of a robust method for the geometric quantification of microtubule bundles in the spindle. In this work, we describe a simple method for measuring and evaluating the shapes of microtubule bundles by characterizing them in terms of their curvature and twist. By using confocal microscopy, we obtain three-dimensional images of spindles, which allows us to trace the entire microtubule bundle. For each traced bundle, we first fit a plane and then fit a circle lying in that plane. With this robust method, we extract the curvature and twist, which represent the geometric information characteristic for each bundle. As the bundle shapes reflect the forces within them, this method is valuable for the understanding of forces that act on chromosomes during mitosis.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
EK-855158 - Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima (ANEUPLOIDY) (Tolić, Iva; Pavin, Nenad, EK ) ( POIROT)
EK-647077 - Nova klasa mikrotubula u vretenu koja djeluje silama na kinetohore (NewSpindleForce) (Tolić, Iva, EK ) ( POIROT)
HRZZ-IP-2019-04-5967 - Ravnoteža sila i momenta sila u diobenom vretenu (SpindleForceBalance) (Pavin, Nenad, HRZZ - 2019-04) ( POIROT)
HRZZ-PZS-2019-02-7653 - Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena (SpindleForm) (Tolić, Iva, HRZZ - 2019-02) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Arian Ivec (autor)

Avatar Url Monika Trupinić (autor)

Avatar Url Iva Tolić (autor)

Avatar Url Nenad Pavin (autor)

Citiraj ovu publikaciju:

Ivec, Arian; Trupinić, Monika; Tolić, Iva M.; Pavin, Nenad
Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles // Biophysical journal, 120 (2021), 17; 3641-3648 doi:10.1016/j.bpj.2021.07.024 (međunarodna recenzija, članak, znanstveni)
Ivec, A., Trupinić, M., Tolić, I. & Pavin, N. (2021) Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical journal, 120 (17), 3641-3648 doi:10.1016/j.bpj.2021.07.024.
@article{article, author = {Ivec, Arian and Trupini\'{c}, Monika and Toli\'{c}, Iva M. and Pavin, Nenad}, year = {2021}, pages = {3641-3648}, DOI = {10.1016/j.bpj.2021.07.024}, keywords = {Mitotic spindle, chirality, twist, helicity, methods}, journal = {Biophysical journal}, doi = {10.1016/j.bpj.2021.07.024}, volume = {120}, number = {17}, issn = {0006-3495}, title = {Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles}, keyword = {Mitotic spindle, chirality, twist, helicity, methods} }
@article{article, author = {Ivec, Arian and Trupini\'{c}, Monika and Toli\'{c}, Iva M. and Pavin, Nenad}, year = {2021}, pages = {3641-3648}, DOI = {10.1016/j.bpj.2021.07.024}, keywords = {Mitotic spindle, chirality, twist, helicity, methods}, journal = {Biophysical journal}, doi = {10.1016/j.bpj.2021.07.024}, volume = {120}, number = {17}, issn = {0006-3495}, title = {Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles}, keyword = {Mitotic spindle, chirality, twist, helicity, methods} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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