engleski

Preliminary study of fatty acid composition of perirenal adipose tissue of free-ranging brown bears from Croatia

There are structural and metabolic differences between adipose depots and it is generally assumed that the fatty acid (FA) composition of all adipose tissue within an individual is the same. Some studies did not detect any site-specific differences, but some consistent differences have been reported. To our knowledge, FA composition of brown bear perirenal adipose tissue (PAT) has not been investigated, so the objective of this study was to determine the FA composition of this tissue. The study was conducted on 18 free-ranging brown bears from Croatia (8 females, F ; 10 males, M) sampled during 2015 hunting season. Tissue samples were homogenized, total lipids extracted than composition of FA was determined by gas chromatography. PAT was dominated by monounsaturated FA (F, 45.5±2.6% vs. M, 43.3±1.7%, p<0.05). Second in the representation were saturated FA (SFA, 42.5±4.8% vs. 44.7±4.5%, p>0.05), while least represented were polyunsaturated FA (PUFA, 3.2±1.9% vs. 2.6±1.2%, p>0.05). Females had significantly lower percentage of C16:2 (1.0±0.3% vs. 3.8±1.2%) compared to males. When compared to our previously published results (F, N=19 ; M, N=57 ; ) regarding FA composition of subcutaneous adipose tissue (SAT), PAT of females had 1.7 times higher representation of SFA and 2.1 times lower representation of PUFA. Furthermore, PAT of males had 1.7 times lower representation of PUFA than SAT of males. Essential C18:2n-6 showed difference in representation in females comparing the two adipose tissues (PAT, 2.4 times lower than SAT). Physiological significance of differences in the FA composition of two adipose tissue locations in the body is possibly dependent on factors such as dietary fat composition, energy balance, and metabolic activity as well as some factors that have to be determined. Regarding this, larger numbers of brown bear PAT samples in different seasons, emphasizing the influence of autumn and spring, have to be investigated.

fatty acid ; perirenal adipose tissue ; brown bear

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