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Pregled bibliografske jedinice broj: 1149674

Agitation induced reversible self-association of protein rHu-G-SCF


(Simpozij studenata doktorskih studija PMF-a) Dončević, Lucija; Brkić, Antun Lovro; Svetličić, Ema; Cindrić, Mario
Agitation induced reversible self-association of protein rHu-G-SCF // PhD Student Symposium 2021 Book of Abstracts / Barišić, Dajana (ur.).
Zagreb: Faculty of Science, University of Zagreb, Croatia, 2021. str. 320-320 (poster, međunarodna recenzija, sažetak, ostalo)


CROSBI ID: 1149674 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Agitation induced reversible self-association of protein rHu-G-SCF

Autori
Dončević, Lucija ; Brkić, Antun Lovro ; Svetličić, Ema ; Cindrić, Mario

Kolaboracija
Simpozij studenata doktorskih studija PMF-a

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
PhD Student Symposium 2021 Book of Abstracts / Barišić, Dajana - Zagreb : Faculty of Science, University of Zagreb, Croatia, 2021, 320-320

ISBN
978-953-6076-90-1

Skup
Simpozij studenata doktorskih studija Prirodoslovno- matematičkog fakulteta

Mjesto i datum
Zagreb, Croatia, 24-25.04.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
protein self-association, agitation, filgrastim

Sažetak
Granulocyte colony-stimulating factor (rHuG-CSF), or so-called filgrastim, is a member of the class of cytokines that stimulates the synthesis of granulocytes in the human body. Filgrastim is structurally composed of 175 amino acids and can be used as a drug for the treatment of neutropenia. While affected by different types of stressors, the monomeric protein form of filgrastim has a tendency to self-associate and produce dimers, trimers, tetramers, and other complex aggregates. A stressor such as heat can induce the formation of irreversible filgrastim aggregates, while agitation stimulates the reversible self-association mechanism. Produced irreversible forms can emerge due to the formation of covalent bonds of monomer protein structures, of which the most significant are disulfide bridges. Further on, reversible filgrastim aggregate forms occur due to the non-covalent bonds, i.e. hydrogen bonds, electrostatic, hydrophobic, and van der Waals interactions. Researchers have discovered that reversible self- associated proteins in the human body can promote the development of Alzheimer's, Parkinson's, Huntington's, and prion's diseases, as well as amyotrophic lateral sclerosis [1], however, the complexity of the filgrastim structure makes the understanding of aforementioned self-association processes extremely challenging. Therefore, to examine the mechanism of the reversible protein binding reaction, the filgrastim aqueous solution was agitated at a constant rate increasing the agitation time by 60-600 s. The analysis was performed immediately after agitation by high performance liquid chromatography (HPLC) and the obtained results were normalized by a mathematical model that derives a more precise quantitative analysis of chromatographic peaks in four steps. Such a mathematical model enabled the illustration of the Lumry-Eyring equation framework that explains the mechanism of an aggregation process of filgrastim.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Lucija Dončević (autor)

Avatar Url Ema Svetličić (autor)

Avatar Url Mario Cindrić (autor)

Avatar Url Antun Brkic (autor)

Poveznice na cjeloviti tekst rada:

www.pmf.unizg.hr

Citiraj ovu publikaciju:

(Simpozij studenata doktorskih studija PMF-a) Dončević, Lucija; Brkić, Antun Lovro; Svetličić, Ema; Cindrić, Mario
Agitation induced reversible self-association of protein rHu-G-SCF // PhD Student Symposium 2021 Book of Abstracts / Barišić, Dajana (ur.).
Zagreb: Faculty of Science, University of Zagreb, Croatia, 2021. str. 320-320 (poster, međunarodna recenzija, sažetak, ostalo)
(Simpozij studenata doktorskih studija PMF-a) (Simpozij studenata doktorskih studija PMF-a) Dončević, L., Brkić, A., Svetličić, E. & Cindrić, M. (2021) Agitation induced reversible self-association of protein rHu-G-SCF. U: Barišić, D. (ur.)PhD Student Symposium 2021 Book of Abstracts.
@article{article, author = {Don\v{c}evi\'{c}, Lucija and Brki\'{c}, Antun Lovro and Svetli\v{c}i\'{c}, Ema and Cindri\'{c}, Mario}, editor = {Bari\v{s}i\'{c}, D.}, year = {2021}, pages = {320-320}, keywords = {protein self-association, agitation, filgrastim}, isbn = {978-953-6076-90-1}, title = {Agitation induced reversible self-association of protein rHu-G-SCF}, keyword = {protein self-association, agitation, filgrastim}, publisher = {Faculty of Science, University of Zagreb, Croatia}, publisherplace = {Zagreb, Croatia} }
@article{article, author = {Don\v{c}evi\'{c}, Lucija and Brki\'{c}, Antun Lovro and Svetli\v{c}i\'{c}, Ema and Cindri\'{c}, Mario}, editor = {Bari\v{s}i\'{c}, D.}, year = {2021}, pages = {320-320}, keywords = {protein self-association, agitation, filgrastim}, isbn = {978-953-6076-90-1}, title = {Agitation induced reversible self-association of protein rHu-G-SCF}, keyword = {protein self-association, agitation, filgrastim}, publisher = {Faculty of Science, University of Zagreb, Croatia}, publisherplace = {Zagreb, Croatia} }




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