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Pregled bibliografske jedinice broj: 1149490

Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth


Marcelić M, Lučin HM, Begonja AJ, Zagorac GB, Lisnić VJ, Lučin P.
Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth // Life, 11 (2021), 8; 859, 26 (međunarodna recenzija, članak, ostalo)


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Naslov
Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth

Autori
Marcelić M, Lučin HM, Begonja AJ, Zagorac GB, Lisnić VJ, Lučin P.

Izvornik
Life (2075-1729) 11 (2021), 8; 859, 26

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
Evectin-2 ; Rab10 ; VPS34-IN1 ; Vps34 ; assembly compartment ; cytomegalovirus ; early endosomes ; endosomal recycling compartment ; phosphatidylinositol-3-phosphate.

Sažetak
Murine cytomegalovirus (MCMV) initiates the stepwise establishment of the pre-assembly compartment (pre-AC) in the early phase of infection by the expansion of the early endosome (EE)/endosomal recycling compartment (ERC) interface and relocation of the Golgi complex. We depleted Vps34-derived phosphatidylinositol-3- phosphate (PI(3)P) at EEs by VPS34-IN1 and inhibited PI(3)P-associated functions by overexpression of 2xFYVE- and p40PX PI(3)P-binding modules to assess the role of PI(3)P-dependent EE domains in the pre-AC biogenesis. We monitored the accumulation of Rab10 and Evectin-2 in the inner pre-AC and the relocation of GM130-positive cis- Golgi organelles to the outer pre-AC by confocal microscopy. Although PI(3)P- and Vps34-positive endosomes build a substantial part of pre-AC, the PI(3)P depletion and the inhibition of PI(3)P- associated functions did not prevent the establishment of infection and progression through the early phase. The PI(3)P depletion in uninfected and MCMV-infected cells rapidly dispersed PI(3)P-bond proteins and reorganized EEs, including ablation of EE-to-ERC transport and relocation of Rab11 endosomes. The PI(3)P depletion one hour before pre-AC initiation and overexpression of 2xFYVE and p40PX domains neither prevented Rab10- and Evectin-2 accumulation, nor Golgi unlinking and relocation. These data demonstrate that PI(3)P-dependent functions, including the Rab11-dependent EE-to-ERC route, are dispensable for pre-AC initiation. Nevertheless, the virus growth was drastically reduced in PI(3)P-depleted cells, indicating that PI(3)P- associated functions are essential for the late phase of infection.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
HRZZ-IP-2020-02-2916 - Reorganizacija sučelja između ranih endosoma i endosomalnog reciklirajućeg odjeljka (CREERC) (Mahmutefendić Lučin, Hana, HRZZ - 2020-02) ( POIROT)
NadSve-uniri-biomed-18-180 - Rab10 u biogenezi endosomalnog reciklirajućeg odjeljka (Mahmutefendić Lučin, Hana, NadSve ) ( POIROT)
NadSve-uniri-biomed-18-88 - Citomegalovirusni odjeljak za sklapanje viriona (Lučin, Pero, NadSve ) ( POIROT)
HRZZ-IP-2019-04-3582 - Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice (AsCoSE&E) (Lučin, Pero, HRZZ - 2019-04) ( POIROT)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-229 - Arf GTPaze u regulaciji endosomalnog prometovanja tijekom citomegalovirusne infekcije (Blagojević Zagorac, Gordana, NadSve ) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka


Citiraj ovu publikaciju:

Marcelić M, Lučin HM, Begonja AJ, Zagorac GB, Lisnić VJ, Lučin P.
Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth // Life, 11 (2021), 8; 859, 26 (međunarodna recenzija, članak, ostalo)
Marcelić M, Lučin HM, Begonja AJ, Zagorac GB, Lisnić VJ, Lučin P. (2021) Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth. Life, 11 (8), 859, 26.
@article{article, year = {2021}, pages = {26}, chapter = {859}, keywords = {Evectin-2, Rab10, VPS34-IN1, Vps34, assembly compartment, cytomegalovirus, early endosomes, endosomal recycling compartment, phosphatidylinositol-3-phosphate.}, journal = {Life}, volume = {11}, number = {8}, issn = {2075-1729}, title = {Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth}, keyword = {Evectin-2, Rab10, VPS34-IN1, Vps34, assembly compartment, cytomegalovirus, early endosomes, endosomal recycling compartment, phosphatidylinositol-3-phosphate.}, chapternumber = {859} }
@article{article, year = {2021}, pages = {26}, chapter = {859}, keywords = {Evectin-2, Rab10, VPS34-IN1, Vps34, assembly compartment, cytomegalovirus, early endosomes, endosomal recycling compartment, phosphatidylinositol-3-phosphate.}, journal = {Life}, volume = {11}, number = {8}, issn = {2075-1729}, title = {Endosomal Phosphatidylinositol-3-Phosphate- Associated Functions Are Dispensable for Establishment of the Cytomegalovirus Pre-Assembly Compartment but Essential for the Virus Growth}, keyword = {Evectin-2, Rab10, VPS34-IN1, Vps34, assembly compartment, cytomegalovirus, early endosomes, endosomal recycling compartment, phosphatidylinositol-3-phosphate.}, chapternumber = {859} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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