Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1149489

Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection


Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P.
Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection // Life, 11 (2021), 8; 867, 26 doi:10.3390/life11080867 (međunarodna recenzija, članak, ostalo)


CROSBI ID: 1149489 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection

Autori
Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P.

Izvornik
Life (2075-1729) 11 (2021), 8; 867, 26

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
Arf GTPases ; Rab10 ; cytomegaloviruses ; m06 ; pre-assembly compartment.

Sažetak
Shortly after entering the cells, cytomegaloviruses (CMVs) initiate massive reorganization of cellular endocytic and secretory pathways, which results in the forming of the cytoplasmic virion assembly compartment (AC). We have previously shown that the formation of AC in murine CMV- (MCMV) infected cells begins in the early phase of infection (at 4-6 hpi) with the pre-AC establishment. Pre-AC comprises membranes derived from the endosomal recycling compartment, early endosomes, and the trans-Golgi network, which is surrounded by fragmented Golgi cisterns. To explore the importance of Arf GTPases in the biogenesis of the pre-AC, we infected Balb 3T3 cells with MCMV and analyzed the expression and intracellular localization of Arf proteins in the early phases (up to 16 hpi) of infection and the development of pre-AC in cells with a knockdown of Arf protein expression by small interfering RNAs (siRNAs). Herein, we show that even in the early phase, MCMVs cause massive reorganization of the Arf system of the host cells and induce the over- recruitment of Arf proteins onto the membranes of pre-AC. Knockdown of Arf1, Arf3, Arf4, or Arf6 impaired the establishment of pre-AC. However, the knockdown of Arf1 and Arf6 also abolished the establishment of infection. Our study demonstrates that Arf GTPases are required for different steps of early cytomegalovirus infection, including the establishment of the pre-AC.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
HRZZ-IP-2020-02-1323 - Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije (Arf & CMV) (Blagojević Zagorac, Gordana, HRZZ - IP-2020-02) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-229 - Arf GTPaze u regulaciji endosomalnog prometovanja tijekom citomegalovirusne infekcije (Blagojević Zagorac, Gordana, NadSve ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P.
Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection // Life, 11 (2021), 8; 867, 26 doi:10.3390/life11080867 (međunarodna recenzija, članak, ostalo)
Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P. (2021) Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection. Life, 11 (8), 867, 26 doi:10.3390/life11080867.
@article{article, year = {2021}, pages = {26}, DOI = {10.3390/life11080867}, chapter = {867}, keywords = {Arf GTPases, Rab10, cytomegaloviruses, m06, pre-assembly compartment.}, journal = {Life}, doi = {10.3390/life11080867}, volume = {11}, number = {8}, issn = {2075-1729}, title = {Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection}, keyword = {Arf GTPases, Rab10, cytomegaloviruses, m06, pre-assembly compartment.}, chapternumber = {867} }
@article{article, year = {2021}, pages = {26}, DOI = {10.3390/life11080867}, chapter = {867}, keywords = {Arf GTPases, Rab10, cytomegaloviruses, m06, pre-assembly compartment.}, journal = {Life}, doi = {10.3390/life11080867}, volume = {11}, number = {8}, issn = {2075-1729}, title = {Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection}, keyword = {Arf GTPases, Rab10, cytomegaloviruses, m06, pre-assembly compartment.}, chapternumber = {867} }

Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font