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Dynamin inhibitors prevent the establishment of the cytomegalovirus assembly compartment in the early phase of infection. (CROSBI ID 299316)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Štimac, Igor ; Jug Vučko, Natalia ; Blagojević Zagorac, Gordana ; Marcelić, Marina ; Lučin Mahmutefendić, Hana ; Lučin, Pero Dynamin inhibitors prevent the establishment of the cytomegalovirus assembly compartment in the early phase of infection. // Life, 11 (2021), 9; 876, 28. doi: 10.3390/life11090876

Podaci o odgovornosti

Štimac, Igor ; Jug Vučko, Natalia ; Blagojević Zagorac, Gordana ; Marcelić, Marina ; Lučin Mahmutefendić, Hana ; Lučin, Pero

engleski

Dynamin inhibitors prevent the establishment of the cytomegalovirus assembly compartment in the early phase of infection.

Cytomegalovirus (CMV) infection initiates massive rearrangement of cytoplasmic organelles to generate assembly compartment (AC). The earliest events, the establishment of the preAC, are initiated in the early phase as an extensive reorganization of early endosomes (EEs), endosomal recycling compartment (ERC), trans-Golgi network (TGN), and the Golgi. Here, we demonstrate that dynamin inhibitors (Dynasore, Dyngo-4a, MiTMAB, and Dynole-34-2) block the establishment of the preAC in murine CMV (MCMV) infected cells. In this study, we extensively analyzed the effect of Dynasore on the Golgi reorganization sequence into the outer preAC. We also monitored the development of the inner preAC using a set of markers that define EEs (Rab5, Vps34, EEA1, and Hrs), the EE- ERC interface (Rab10), the ERC (Rab11, Arf6), three layers of the Golgi (GRASP65, GM130, Golgin97), and late endosomes (Lamp1). Dynasore inhibited the pericentriolar accumulation of all markers that display EE-ERC-TGN interface in the inner preAC and prevented Golgi unlinking and dislocation to the outer preAC. Furthermore, in pulse-chase experiments, we demonstrated that the presence of dynasore only during the early phase of MCMV infection (4-14 hpi) is sufficient to prevent not only AC formation but also the synthesis of late-phase proteins and virion production. Therefore, our results indicate that dynamin-2 acts as a part of the machinery required for AC generation and rearrangement of EE/ERC/Golgi membranes in the early phase of CMV infection.

Golgi apparatus ; assembly compartment ; cytomegalovirus ; dynamin ; early endosomes ; endosomal recycling compartment.

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Podaci o izdanju

11 (9)

2021.

876

28

objavljeno

2075-1729

10.3390/life11090876

Povezanost rada

Kliničke medicinske znanosti

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