engleski

THE EFFECT OF 2500 MHZ MICROWAVE RADIATION ON THE SPERM MOTILITY AND VELOCITY PARAMETERS OF IN VITRO IRRADIATED SEMEN OF BREEDING BOARS

With the development of technology, there is an increase in public concern about the negative effects of radiofrequency electromagnetic radiation (RF-EMR), especially of device that operates at a frequency of 2500 MHz. It is presumed that the male reproductive system could be one of the most affected by negative effects. The data on the effect of RF-EMZ on the reproductive system of male domestic animals are scarce. The aim of this study was to investigate the effect of electromagnetic radiation with a frequency of 2500 MHz on sperm motility and velocity parameters of in vitro irradiated semen of breeding boars. The ejaculates of 12 breeding boars, Landrace and Pietrain breed, aged 1.5 to 3.5 years were sampled by manual fixation of the penis. Freshly diluted semen of each boar were divided into a control (n = 12) and an experimental sample (n = 12) that was irradiated. The experimental samples were exposed to continuous non-ionizing electromagnetic radiation of 2500 MHz in gigahertz transverse electromagnetic chamber for 2 hours at an electromagnetic field strength of 10 V/m. In the control and experimental groups, the motility and sperm velocity parameters were determined using computer assisted sperm analyser. Following sperm velocity parameters were analysed: curvilinear velocity, straight-line velocity, average path velocity, amplitude of lateral head displacement, linearity, wobble, straightness and beat cross frequency. Significant decrease (p<0.001) in the proportion of progressive sperm motility was recorded in the experimental group. Also, in the experimental group a significant decrease in the share of progressive motile sperm, in the share of the straightness, in the share of the linearity index, in the values of the straight-line velocity and the beat cross frequency was recorded. According to the results of this study, it can be concluded that EMR frequency of 2500 MHz has a negative effect on sperm motility and velocity parameters in irradiated semen of breeding boars.

2500 MHz microwave radiation, sperm motility, velocity parameters in vitro irradiated semen, breeding boars

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