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Qualitative and quantitative analysis of polyphenols of Micromeria kosaninii Šilić (Lauraceae)

INTRODUCTION AND OBJECTIVE The genus Micromeria Benth includes many endemic and sub-endemic species that are poorly represented in traditional medicine due to their limited distribution and inaccessible sites. So it is not surprising that today it attract the particular attention of scientists as poorly explored potential source of medicinal substances. Micromeria kosaninii Šilić is a perennial plant from the Lamiaceae family which grows in rock crevices in the mountain regions of Macedonia, and in 2013 was also found in the National Park Prespa in Albania [1]. In this study, phytochemical analysis of polyphenolic compounds from aerial parts of the Macedonian endemic species M. kosaninii (Lamiaceae) was performed. METHODS Qualitative analysis of polyphenolic compounds in methanolic extract of M. kosaninii was performed by chemical reactions and by thin layer chromatography (TLC) [2, 3]. The content of polyphenolic substances was determined by various spectrophotometric methods: phenolic acids (PA ; A505nm and A525nm), flavonoids (F ; A425nm), and total polyphenols and tannins (TP and T ; A720nm) [4]. RESULTS The presence of polyphenols in the methanolic extract of M. kosaninii was confirmed by the chemical reactions of the formation of characteristic colored products and precipitates. TLC analysis determined the presence of flavonoids and phenolic acids: naringin, quercitrin and isoquercitrin, as well as chlorogenic, isochlorogenic, caffeic and rosmarinic acids. Quantitative spectrophotometric analysis of polyphenols revealed the following: the PA content (expressed as rosmarinic acids) was 4.30%±0.21% (A505 nm), while the PA content expressed as chlorogenic acid was 7.97±0.37 ; the content of F was 0.07±0.01% ; the TP content was determined in concentration of 8.62±0.13%, while the content of T was 2.76±0.15%. CONCLUSIONS The performed phytochemical characterization represents a scientific contribution to the current research of the genus Micromeria and the Macedonian endemic plant species, and also completes the existing knowledge about biological activity and phytotherapeutic potential of the species Micromeria kosaninii.

total polyphenols ; phenolic acids ; flavonoids ; tannins ; Micromeria kosaninii

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