engleski

DIETARY n-3 PUFAs INCREASE ANTI-INFLAMMATORY CYTOKINE TGF-β1 PRODUCTION BY PBMCs FOLLOWING PMA/IONOMYCIN STIMULATION

Introduction: Fatty acid derived lipid mediators have an important role in maintaining cytokine environment through direct effects on cytokine production and T lymphocytes. In the present study, we aimed to determine the effects of n-3 PUFA supplementation (~1053 mg/per day) through consumption of enriched hen eggs, on pro- and anti-inflammatory markers in healthy individuals (N=40 ; 23.8 ± 2.57 years old). Materials and Methods: Participants consumed three regular (N=21) or enriched hen eggs (N=19) per day through three weeks. Mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation and cultured in RPMI-1640 media (10% FBS ; 1% antibiotic). Concentrations of anti-/pro-inflammatory cytokines (IL-10, IL-6, IL- 23, IL-17A, and TGF-β1) and MCP-1 chemokine in both control and n-3 PUFA group, before and after dietary protocols were determined in supernatants following PMA/ionomycin stimulation with Invitrogen ProcartaPlex antibody-based, magnetic bead reagent kits on the Luminex 200 instrument platform. Results: TGF-β1 production by PBMCs following n-3 PUFA dietary protocol was significantly increased (p=0.026), while IL-6 production was significantly decreased (p=0.05), compared to the respective baseline levels. Target cytokine and chemokine production was unaffected by the consumption of regular hen eggs. Conclusion: The results of the present study have demonstrated that dietary n-3 PUFAs can modify key markers of inflammation and lead towards inflammation resolving environment in the population of young, healthy individuals.

inflammation, functional food, PBMCs, cytokines

The study was funded by European Structural and Investment Funds to Science Centre of Excellence for Personalised Health Care, the Josip Juraj Strossmayer University of Osijek, Scientific Unit for Research, Production and Medical Testing of Functional Food, # KK.01.1.1.01.0010.