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Protective role of flavonoids incorporated into DOPC liposomes upon initiated lipid peroxidation

Structural properties of lipid membranes, such as integrity, elasticity, and fluidity are critical for cellular activities. One of the main components of biological membrane are unsaturated lipids, which are particularly susceptible to peroxidation by reactive oxygen species due to highly reactive hydrogen atom on the methylene bridge. The peroxidation of unsaturated lipids, in our case 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces structural reorganization of the membrane. Flavonoids, polyphenolic biomolecules with antioxidative activity, show great potential for the protection of the lipid membrane. Interactions of flavonoids with membranes exposed to the oxidative stress have been investigated with the respect to their influence on the membrane structure, elasticity, and functionality. Multiple methods have been used to investigate the interaction of flavonoids with model cell membranes. The calculation of the percentage inhibition was carried out using infrared spectroscopy (ATR technique) by monitoring the change in the carbonyl stretching band. Mass spectrometry was used to identify some products of lipid peroxidation reaction and to gain insight into the mechanism which takes place. In addition, atomic force microscopy (AFM) measurements were performed to gain more insight into protection of the membrane during the reaction. The aim of this study is to use complementary methods to elucidate the mechanism and the products of the lipid peroxidation reaction, as well as to study protective role of flavonoids which were incorporated into model lipid membranes in different molar ratios.

antioxidative activity ; flavonoids ; lipid peroxidation

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