engleski

Malignant transformation of ganglioglioma to anaplastic ganglioglioma: a ganglioside characterization study

Anaplastic gangliogliomas (AGGLs) are rare, neuronal-glial types of tumors classified as WHO grade III, strongly associated with short overall survival rate. AGGLs are composed of dysplastic ganglion cells and anaplastic glial component. Although AGGLs occasionally occur de novo, benign gangliogliomas (WHO grade I) can develop a malignant transformation into AGGLs. Gangliosides are sialylated glycosphinoglipids highly abundant in neural cells where they act as functional and structural components of cell membrane. Changes in their composition are recognised as a hallmark of malignant transformation and they strongly correlate with tumor growth and invasiveness. Herein, we present a rare case of transformation from a low- malignancy ganglioglioma to AGGL and characterization of ganglioside expression in AGGL, peritumoral (PT) and normal brain (NB) tissue. Complex ganglioside mixtures were isolated and purified from AGGL, PT and NB tissue in parallel. The total ganglioside content was spectrophotometrically determined and ganglioside fractions were characterized by a high performance thin-layer chromatography (HPTLC). The total ganglioside content in AGGL was approximately six times lower than in NB tissue and five times lower than in PT tissue.The ganglioside pattern of HPTLC-separated fractions showed a highly altered composition of ganglioside species in AGGL compared to PT and NB tissue. The ganglioside composition in PT tissue was highly similar to NB tissue. The ganglioside composition in AGGL was highly distinguishable from both PT and NB tissue. The ganglioside characterization elucidates a realistic state of ganglioside expression at the cell surface identifying the major ganglioside species acting as cell-surface antigens of specific tumor specimens.

gangliosides ; anaplastic ganglioglioma ; malignant transformation ; ganglioglioma ; high performance thin-layer chromatography

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