engleski

Genotoxic properties of low doses of cadmium and phtalates

Cadmium and phtalates are often found as environmental contaminats. Cadmium enters the food chain both naturally and from industrial and agricultural sources. Foodstuffs are the main source of cadmium exposure for the non-smoking general population (cereal products, vegetables, nuts and pulses, starchy roots or potatoes, and meat products). Phtalates are not directly acting genotoxic compounds but are associated with a variety of health outcomes. Diet is considered a significant exposure pathway of these compounds. Poultry, cooking oils, and cream-based dairy products are the main food groups in which these compounds can be found. In this presentation, the genotoxic potential of both compounds will be presented. Different human cell lines will be used as biological test systems ; human larynx carcinoma (HEp-2) as a representative of the epithelial cells which are first in contact with these contaminants after ingestion ; human colon carcinoma (Caco-2) cells, and human hepatocellular carcinoma (HepG2) cells in which metabolic reactions and presence of certain endogenous compounds can influence the overall (geno)toxicity of investigated compounds. Ames strains Salmonella typhimurium Ta98 and TA100 are useful in the determination of the potential of these two chemicals to cause point mutatations.

environment contaminants ; genotoxicity ; human cell lines ; point mutations ; test systems

nije evidentirano