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Influence of bearberry extract (Arctostaphylos uva ursi L.) on gastrointestinal bacteria adhesion

Bearberry extract has been used for centuries in traditional and modern medicine to treat and alleviate the problems caused by urinary infections. Nevertheless, the exact mechanism of action of biologically active compounds from bearberry extract to date has not been fully defined. In this thesis the cytotoxicity effect of bearberry water extract, including the comparison between fresh and freeze-dried extract effects on bacteria Escherichia coli, Staphylococcus aureus, Lactobacillus plantarum and Lactobacillus fermentum was studied. Furthermore, the influence of the extract on adhesion of aforementioned bacteria to human epithelial gastrointestinal tract cells in the in vitro system was studied. Before the adhesion tests, cytotoxicity tests using Neutral red method were carried out together with the tests of potential antioxidant activity of the extract using DCHF-DA method on human tongue squamous cell carcinoma cell line (CAL 27) and human epithelial colorectal adenocarcinoma cells (Caco-2). The results obtained show a strong antimicrobial effect of this extract towards Gram-positive bacteria and also its ability to cause changes in the adhesion of bacterial cells to the epithelial cells of the digestive system. Antioxidant activity of the extract was established and stimulation of human cell proliferation was determined, which depends on the concentration of the extract and the type of treated cells. No significant difference was observed by comparing the biological activity of freshly prepared and freeze-dried bearberry water extract.

antioxidant activity ; Caco-2 ; CAL 27 ; toxicity ; urinary infection

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