engleski

Characterization of human tau protein expressed in quiescent yeast

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of tau protein aggregates in cells of the affected brain regions and the consequent neuronal death. In healthy neurons, tau is a soluble, intrinsically disordered protein that is mostly bound to the microtubules in the axon, however, in the affected neurons, tau accumulates in the soma and dendrites within amyloid-like aggregates, presumably via an intermediary step involving toxic oligomeric structures. The main risk factor for the onset of AD is aging, however, despite a vast number of studies, the causes of tau protein aggregation and toxicity are still largely unclear. Since the ability of a cell to maintain protein homeostasis decreases with aging, impaired protein quality control pathways are considered a possible factor in the development of AD. Yeast is a single cell eukaryotic organism that is amenable to genetic analysis, and many of its cellular pathways, including protein quality control are evolutionarily conserved. Moreover, quiescent yeast can be used to study processes characteristic for non-dividing cells, such as chronological aging. To investigate factors that influence tau protein aggregation and toxicity, in particular cellular aging and proteotoxic stress, we expressed human tau protein in yeast Saccharomyces cerevisiae. We will present a characterization of human tau protein expressed in quiescent yeast. Acknowledgments: Supported by the Research Cooperability Program of the Croatian Science Foundation funded by the European Union from the European Social Fund under the Operational Program Efficient Human Resources 2014-2020 (grant PZS-2019-02-3610) ; Croatian Science Foundation grants IP-2019-04-3584 and DOK- 01-2018/ European Social Fund ; Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic-ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund).

aging ; Alzheimer's disease ; Saccharomyces cerevisiae ; non-dividing cells ; protein aggregation ; tau protein expression ; yeast

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