Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines (CROSBI ID 70733)
Prilog u knjizi | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Heger, Lukas ; Lühr, Jennifer J. ; Amon, Lukas ; Smith, Ana-Sunčana ; Eissing, Nathalie ; Dudziak, Diana
engleski
Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines
Confocal immunofluorescence microscopy is an advanced imaging technique routinely applied in the laboratory and clinics. Histological analyses are performed from tissue material. In general, a single fluorochrome per laser is employed, limiting simultaneous analysis to four antigens in one staining with a conventional 4-laser line microscope. Here, we describe a protocol for combining fluorochromes with the same excitation but different emission properties that allows for the analysis of six different antigens in confocal immunofluorescence microscopy with a conventional 4-laser line microscope. The proposed multiplexed method permits the identification and characterization of complex cell populations in rare tissue material.
Multicolor imaging ; Fluorochrome ; Tissue Histology ; Dendritic cells ; DC
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Podaci o prilogu
21-30.
objavljeno
10.1007/978-1-0716-1593-5_2
Podaci o knjizi
Multiplexed Imaging Methods and Protocols
Eli, Zamir
New York (NY): Springer
2021.
978-1-0716-1592-8
1064-3745
1940-6029