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Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: Evaluation of DPPH free radical scavenging activity by HPLC

Soybeans, red clover, chastetree, hop and flax have all been found to contain a wide range of phytoestrogenic compounds, and a large number of dietary supplements contain their extracts as principal ingredients. Since polyphenolic compounds are responsible for the potential antioxidant activity and radical scavenging capacity of plant food, this study aimed to evaluate total polyphenol content (TPC) and antioxidant activity (AA) of phytoestrogen- containing food and dietary supplement (DS) formulated products prepared. The HPLC-DPPH method was applied for the analysis of various phytoestrogen-containing samples. Polyphenol content and antioxidant activity in formulated DS (TPC: 17.65±10.28 mg/g ; AA: 103.01±58.42 mM TROLOX/g) was higher than in functional food samples (TPC: 6.18±1.37 mg/g dry matter ; AA: 40.01±15.80 mM TROLOX/g dry matter). Also, the results indicate that multi-botanical products (TPC: 23.70±9.09 mg/g ; AA: 143.49±45.63 mM TROLOX/g) have higher polyphenol content and antioxidant activity than monopreparation (TPC: 15.63±10.11 mg/g ; AA: 89.52±57.07 mM TROLOX/g). Furthermore, the correlation between polyphenol content and the antioxidant activity was strongly statistically significant, so it can be concluded that antioxidant activity is proportional to the content of secondary metabolites. The most eye- catching batch-to-batch deviations were represented by two chasteberry-based products (RSD 12.6% and 41.3%) and one red clover (RSD 57.9%) derived product.

phytoestrogens ; polyphenol content ; antioxidant activity ; functional food ; dietary supplements

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