engleski

Tar DNA-binding protein 43 regulation in mice spinal cord during the first week following repetitive mild traumatic brain injury

Introduction: Repetitive mild traumatic brain injury (mTBI) is a risk factor for the development of different neurodegenerative diseases, including amyotrophic lateral sclerosis which is characterized by TAR DNA-binding protein 43 (TDP- 43) proteinopathy in most cases. The aim of this research was to examine intracellular regulation of TDP-43 in the spinal cord during the first week after repetitive mTBI in mice. Materials and methods: Repetitive mTBI was done by the weight drop method (Kane et al. 2012) twice a day, for five consecutive days in C57BL/6 mice. The control group of mice was subjected to a sham procedure, had the same handling and anesthesia, but received no impact. The mice were sacrificed one, three or seven days after the final injury/sham procedure and their spinal cords were dissected and prepared for the Western blot analyses of proteins of interest. Results: In the spinal cords of injured mice, increased cytoplasmatic expression of the phosphorylated TDP-43 was detected on the first day after repetitive mTBI, while the expression of TDP-43 and its cleavage product TDP-35, as well as of the phosphorylated TDP-35 and TDP-25 were not impaired at any of time points investigated. Conclusions: Our preliminary results suggest early and transient dysregulation of TDP-43 in the cytoplasm of the spinal cord cells in mice following repetitive mTBI, which are probably the result of an acute response to stress caused by the brain trauma.

Brain Concussion ; Mice ; TDP-43 Proteinopathies

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