Redox‐active cysteines in TGACG‐BINDING FACTOR 1 (TGA1) do not play a role in salicylic acid or pathogen‐induced expression of TGA1‐regulated target genes in Arabidopsis thaliana (CROSBI ID 298912)
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Budimir, Jelena ; Treffon, Katrin ; Nair, Aswin ; Thurow, Corinnna ; Gatz, Christiane
engleski
Redox‐active cysteines in TGACG‐BINDING FACTOR 1 (TGA1) do not play a role in salicylic acid or pathogen‐induced expression of TGA1‐regulated target genes in Arabidopsis thaliana
Salicylic acid (SA) is an important signaling molecule of the plant immune system.In Arabidopsis thaliana, SA biosynthesis is indirectly modulated by the closely related tran- scription factors TGACG-BINDING FACTOR 1 and 4 (TGA1 and TGA4, respectively). Theyactivate expression of SYSTEMIC ACQUIRED RESISTANCE DEFICIENT1, the gene product ofwhich regulates the key SA biosynthesis gene ISOCHORISMATE SYNTHASE 1.Since TGA1 interacts with the SA receptor NONEXPRESSOR OF PATHOGENESIS-RELATEDGENES 1 (NPR1) in a redox-dependent manner and since the redox state of TGA1 is altered inSA-treated plants, TGA1 was assumed to play a role in the NPR1-dependent signaling cas-cade. Here, we identified 193 out of 2090 SA-induced genes that require TGA1/TGA4 formaximal expression after SA treatment. One robustly TGA1/TGA4-dependent gene encodesfor the SA hydroxylase DOWNY MILDEW RESISTANT 6-LIKE OXYGENASE 1, suggesting anadditional regulatory role of TGA1/TGA4 in SA catabolism.Expression of TGA1/TGA4-dependent genes in mock/SA-treated or Pseudomonas-infectedplants was rescued in the tga1 tga4 double mutant after introduction of a mutant genomicTGA1 fragment encoding a TGA1 protein without any cysteines. Thus, the functional signifi-cance of the observed redox modification of TGA1 in SA-treated tissues remains enigmatic
Arabidopsis thaliana ; defense responses ; NPR1 ; redox regulation ; salicylic acid ; TGA transcription factors
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