engleski

Laminar dynamics of CELF1 expression in early and mid fetal human cerebral wall

Precise regulation of gene expression is a prerequisite for normal brain development. Unlike regulation of transcription, which has been studied for decades, the importance of regulating mRNA translation during prenatal brain development has been studied only recently. Here we studied laminar dynamics of CELF1 expression pattern in prenatal human cerebral wall, as it was previously shown that Celf1 is required for the development of early neuronal progenitors and glutamatergic neurons during the mouse corticogenesis [1]. Dynamics of CELF1 expression in human brain is less known. The development of the prenatal human brain is characterized by the appearance of temporary fetal zones essential for the establishment of the proper organization of the future cerebral cortex. The dynamics of brain development, time course, and spatial organization are controlled by the regulation of gene expression at several levels. A number of factors are involved in posttranscriptional and translational regulation, including RNA-binding proteins (RBPs). The role of RNA-binding proteins is to control gene expression by creating ribonucleo-protein complexes. RBPs are involved in the regulation of neurogenetic processes, such as proliferation, cell specification, migration, and neuronal maturation [2]. Therefore, we analyzed CELF1 expression pattern utilizing immunofluorescence throughout prenatal postmortem human brain tissue in order to reveal laminar shifts and molecular specification of major classes of neurons. Our results showed the expression dynamics of RNA binding protein CELF1 during the early and mid fetal period. In addition we performed CELF1 double labelling with the upper cortical layer-enriched markers such as SATB2, CUX1, CUX2 and the lower cortical layer-enriched markers, such as TLE4, CTIP2, SOX5, TBR1. Accordingly, our results suggest CELF1 involvement in various neurogenetic processes during human corticogenesis such as neuronal identity, their molecular specification and laminar destination.

RNA binding proteins ; human brain development ; CELF1

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