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Elucidating the bioactive potential of mountain germander (Teucrium montanum) by applying fractionation of phenolic compounds

The inclusion of herb plants in folk medicine has been present through the decades in frame of its health benefits. Emerging from the presence of biologically active compounds, Mountain Germander represents insufficiently explored plant with widespread interest in the pharmacological investigations and possible findings applications in food industry. In order to closely deliver an insight into the polyphenolic content of Mountain Germander, free- soluble (FP), esterified (EP) glycosylated (GP) and insoluble- bound (IP) phenolic fractions were prepared and analysed using spectrophotometric and HPLC- PDA methodology. FP, EP and IP fractions exhibited similar total phenolic content and antioxidant capacity. Initial extract showed to be the noteworthy source of echinacoside (7.05±0.03 mg/g dw) and verbascoside (4.94±0.09 mg/g dw). Caffeic esters were the most dominant in EP fraction (3.09±0.52 mg/g dw) as the consequence of the retained echinacoside and verbascoside in the aqueous phase, which is in accordance to the absence of echinacoside and lower content of verbascoside (1.87±0.30 mg/g dw) in FP fraction. Notable content of p-coumaric acid was determined in EP (1.38±0.22 mg/g dw) and IP (1.59±0.00 mg/g dw) fractions. This findings could lead toward expanded comprehension of overall bioactive composition of T. montanum, as promising source of functional agents.

Mountain Germander (T. montanum) ; fractionation ; esterified phenolics ; HPLC analysis

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