engleski

Spectroscopic studies of mitochondria targeting multiply charged DABCO-cyanine dyes

Within the last two decades novel design of non- covalent recognition of DNA/RNA by small molecules very often combines two or more non-covalent binding modes (intercalation, groove binding, or electrostatic sugar-phosphate backbone binding) [1] aiming for new dyes selectively reporting on structural differences of DNA and RNA secondary structures. Within this context, aromatic cationic dyes with the ability to penetrate through the cell membranes are of great interest. In general, the tendency of lipophilic cationic dyes is to retain preferentially in the mitochondrial space, which renders them in a particularly important group of fluorescent probes for the study of the mitochondrial lipid bilayer, membrane- permeability, and specific staining of these organelles. Encouraged by our previous research efforts in the design of specific mitochondrial fluorescent probes based on benzoxazolium and benzothiazolium dicationic monomethine cyanine dyes [1-3], we have prepared new series of lipophilic cyanine dyes equipped with several cationic quaternary ammonium moieties. Spectroscopic and isothermal titration calorimetry studies as well as MTT assay and subcellular localization have revealed that presented dyes combine very low cytotoxicity with efficient cellular uptake and remarkable fluorescent marking of mitochondria.

Cyanine dyes ; DABCO ; mitochondrial dyes ; spectroscopy

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