engleski

Development of biocatalytic reactions – an engineering approach

Biocatalytic reactions provide great opportunities in the synthesis of novel compounds and greener ways to produce the already existing ones [1, 2], which is important nowadays when we want to be less dependent upon non-renewable resources. Still, to broaden the industrial application of biochemical reactions, there are many hurdles along the way. These require resolving by an integral and interdisciplinary approach including organic chemistry, enzyme, medium, reaction and process engineering etc. [3]. The latter two approaches enable choosing the right reactor mode, as well as optimization of the reaction conditions that facilitate obtaining the process metrics goals such as product concentration and yield, volume productivity and biocatalyst yield. Considering that these process values are of crucial importance for evaluation of process viability, they represent an important milestone in any process development, on lab scale and beyond [4]. In this lecture, a special focus is placed on process/reaction engineering application in complex biocatalytic processes [5]. This area of research, even though growing in the last decade, is still not applied enough in the development of biocatalytic processes. Advantages of their applications will be presented based on experiences in our research group. [1] S. Wu, R. Snajdrova, J.C. Moore, K. Baldenius, U.T. Bornscheuer, Biocatalysis: Enzymatic Synthesis for Industrial Applications, Angew. Chem. Int. Ed. 2021, 60, 88 –119. [2] E.L. Bell, W. Finnigan, S.P. France, A.P. Green, M.A. Hayes, L.J. Hepworth, S.L. Lovelock, H. Niikura, S. Osuna, E. Romero, K.S. Ryan, N.J. Turner, S.L. Flitsch, Biocatalysis, Nature reviews, Methods Primers (2021) 1:46. [3] R.A. Sheldon, P.C. Pereira, Biocatalysis engineering: the big picture, Angew. Chem. Int. Ed. 2021, 60, 88–119. [4] P. Tufvesson, J. Lima-Ramos, M. Nordblad, J.M. Woodley, Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes, Organic Process Research & Development 2011, 15, 266–274. [5] M. Sudar, Z. Findrik Blažević, Enzyme Cascade Kinetic Modelling, Enzyme Cascade Design and Modelling, S. Kara, F. Rudroff (Eds.), Switzerland: Springer International Publishing, 2021. p 91-108.

kinetic model ; experimental design ; enzyme reactions

nije evidentirano