engleski

Deep eutectic solvents in the extraction of bioactive compounds from two brown algae padina pavonica and cystoseira compressa

Marine algae are known for their numerous benefits and pharmacological effect due to the presence of various bioactive compounds such as terpenoids, meroterpenoids, lipids, phlorotannins and phenolic compounds. Phlorotannins usually serve as a plant defense system against different adverse conditions and are often associated with different biological activities of macroalgae extracts, such as antifungal against Candida sp., antibacterial, anti-inflammatory, antioxidant, antitumor and neuroprotective. Phlorotannins possess numerous - OH groups in their structure, making them a highly hydrophilic molecules which can be extracted using polar deep eutectic solvents (DES).Fifteen different choline chloride based DESs were used for extraction of phlorotannins from Padina pavonica and Cytoseira compressa. Phlorotanin content and antioxidant activity (DPPH method) were determined spectrophotometrically in obtained extracts. Phlorotannines content is much higher in most of the C. compressa extracts compared to P. pavonica. For C. compressa, choline chloride:lactic acid (1:2) showed the best results, considering both phlorotannin yield and DPPH scavenging activity. In the case of P. pavonica extracts, choline chloride:urea (1:2) was the most efficient for the extraction of phlorotannins and the best DPPH scavenging activity was obtained with choline chloride:thiourea (1:2).

brown algae ; extraction ; deep eutectic solvents ; stephlorotannins ; DPPH

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