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Pregled bibliografske jedinice broj: 1144808

Emerging noncanonical functions of plant aminoacyl- tRNA synthetase


Rokov Plavec, Jasmina; Kekez, Mario; Ević, Valentina; Zanki, Vladimir; Šoić, Ružica; Matković-Čalogović, Dubravka; Kekez, Ivana
Emerging noncanonical functions of plant aminoacyl- tRNA synthetase // FEBS Open Bio, vol. 11, issue S1, Supplement: The 45th FEBS Congress: Molecules of Life: Towards New Horizons
Ljubljana, Slovenija, 2021. str. 14-14 doi:10.1002/2211-5463.13206 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1144808 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Emerging noncanonical functions of plant aminoacyl- tRNA synthetase

Autori
Rokov Plavec, Jasmina ; Kekez, Mario ; Ević, Valentina ; Zanki, Vladimir ; Šoić, Ružica ; Matković-Čalogović, Dubravka ; Kekez, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS Open Bio, vol. 11, issue S1, Supplement: The 45th FEBS Congress: Molecules of Life: Towards New Horizons / - , 2021, 14-14

Skup
The 45th FEBS Congress: Molecules of Life: Towards New Horizons

Mjesto i datum
Ljubljana, Slovenija, 03-08.07.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
seryl-tRNA synthetase, BEN1, brassinosteroid, disulfide link

Sažetak
Aminoacyl-tRNA synthetases (aaRSs) participate in translation, catalyzing formation of aminoacyl- tRNAs. Many aaRSs are involved in cellular processes beyond translation but reports on noncanonical functions of plant aaRSs are scarce. We have identified protein BEN1 as protein interactor of seryl-tRNA synthetase (SerRS) from plant Arabidopsis thaliana. BEN1 is involved in metabolism of brassinosteroid hormones that regulate variety of physiological processes, including stress response. Interaction interface involves SerRS globular catalytic domain and N- terminal extension of BEN1. The partnership between SerRS and BEN1 indicates a link between protein translation and steroid metabolic pathways of the plant cell. Structural studies revealed that Arabidopsis SerRS contains intrasubunit disulfide bridge. Cysteines (at positions 213 and 244) involved in disulfide link are conserved in all SerRSs from green plants, indicating their plant- specific functional importance. In order to determine whether disulfide link plays structural or alosteric role we have substituted cysteines with serine. C213S mutant showed lower stability compared to the wild type. Unexpectedly, C244S mutant showed higher stability than wild type, while double mutant had the same stability as the wild type. Crystal structure of C244S mutant and modeled structures of two other mutants showed that number of hydrogen bonds involving residues at postions 213 and 244 correlated with protein stability. The results imply that cysteines involved in the disulfide link are important but not essential for SerRS stability. Future studies are aimed at determination of enzyme activity of the wild type and mutant proteins. Considering that disulfide bonds in cytosolic proteins are usually linked to response mechanisms to oxidative stress, the disulfide link in plant SerRSs may be involved in regulation of translation under oxidative stress conditions.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-6272 - Aminoacil-tRNA-sintetaze kao čuvari standardnog genetičkog koda (AARSCODE) (Gruić Sovulj, Ita, HRZZ - 2016-06) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi febs.onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Rokov Plavec, Jasmina; Kekez, Mario; Ević, Valentina; Zanki, Vladimir; Šoić, Ružica; Matković-Čalogović, Dubravka; Kekez, Ivana
Emerging noncanonical functions of plant aminoacyl- tRNA synthetase // FEBS Open Bio, vol. 11, issue S1, Supplement: The 45th FEBS Congress: Molecules of Life: Towards New Horizons
Ljubljana, Slovenija, 2021. str. 14-14 doi:10.1002/2211-5463.13206 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Rokov Plavec, J., Kekez, M., Ević, V., Zanki, V., Šoić, R., Matković-Čalogović, D. & Kekez, I. (2021) Emerging noncanonical functions of plant aminoacyl- tRNA synthetase. U: FEBS Open Bio, vol. 11, issue S1, Supplement: The 45th FEBS Congress: Molecules of Life: Towards New Horizons doi:10.1002/2211-5463.13206.
@article{article, author = {Rokov Plavec, Jasmina and Kekez, Mario and Evi\'{c}, Valentina and Zanki, Vladimir and \v{S}oi\'{c}, Ru\v{z}ica and Matkovi\'{c}-\v{C}alogovi\'{c}, Dubravka and Kekez, Ivana}, year = {2021}, pages = {14-14}, DOI = {10.1002/2211-5463.13206}, keywords = {seryl-tRNA synthetase, BEN1, brassinosteroid, disulfide link}, doi = {10.1002/2211-5463.13206}, title = {Emerging noncanonical functions of plant aminoacyl- tRNA synthetase}, keyword = {seryl-tRNA synthetase, BEN1, brassinosteroid, disulfide link}, publisherplace = {Ljubljana, Slovenija} }
@article{article, author = {Rokov Plavec, Jasmina and Kekez, Mario and Evi\'{c}, Valentina and Zanki, Vladimir and \v{S}oi\'{c}, Ru\v{z}ica and Matkovi\'{c}-\v{C}alogovi\'{c}, Dubravka and Kekez, Ivana}, year = {2021}, pages = {14-14}, DOI = {10.1002/2211-5463.13206}, keywords = {seryl-tRNA synthetase, BEN1, brassinosteroid, disulfide link}, doi = {10.1002/2211-5463.13206}, title = {Emerging noncanonical functions of plant aminoacyl- tRNA synthetase}, keyword = {seryl-tRNA synthetase, BEN1, brassinosteroid, disulfide link}, publisherplace = {Ljubljana, Slovenija} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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